Food and Feed Antibiotic Chloramphenicol ELISA Test Kit

【Introduction】 

Chloramphenicol is a broad-spectrum antibiotic which is frequently employed in animal production for its excellent antibacterial and pharmacokinetic properties. However, in humans it leads to hematotoxic side effects, in particular chloramphenicol-induced aplastic anaemia for which a dosage-effect relationship has not yet been established. This has led to a prohibition of chloramphenicol for the treatment of animals used for food production.

【Principle of Test】 

Chloramphenicol ELISA Test Kit is an indirect competitive enzyme-labeled immunoassay. The chloramphenicol antigen is precoated on the wells. The precoated antigen compete the chloramphenicol antibody (antibody solution) with sample chloramphenicol in the, anti-chloramphenicol antibody binds to the chloramphenicol-HRP enzyme conjugate. Then pipet the substrate solutions to the wells to convert the color.

The color of unknown samples is compared to the color of the standards and the chloramphenicol concentration of the samples is derived.

【Cross-reactivity】

Chloramphenicol……………………………………………100%

Chloramphenicol Base……………………………………………0.5%

Methyl Chloramphenicol………………………………………≤0.05%

Florfenicol……………………………………………………… <0.01%

Florfenicol Amine………………………………………………..<0.1%

【Materials Required But Not Provided】

1.  Devices:

—- Microwell Plate Reader (450nm/630nm)

—- Vortex

—- Centrifuge

—- Constant Temperature Incubator / Water bath

—- Termovap Sample Concentrator

—- Balance (sensibility: 0.01g)

—- Rubber suction bulb

—- Graduated pipette: 10mL

—- Centrifuge tube: 7mL / 10mL, 50mL

—- Micropipette: Single channel pipet (10µL-100µL, 100µL-1000µL)

Multi-channel pipet (30-300µL)

2. Reagent:

—- Deionized water

—- Ethyl acetate (AR)

—- N-hexane (AR)

—- Sodium carbonate (Na₂CO₃) (AR)

—- Sodium hydrogen carbonate (NaHCO₃) (AR)

—- 18M concentrated sulfuric acid (H₂SO₄)

【Provided Materials and Reagent】

Note: The concentrations of the standards are 0μg/kg, 0.01μg/kg, 0.03μg/kg, 0.09μg/kg, 0.27μg/kg and 0.81μg/kg.