Background:
Fumonisin is one of the mycotoxins produced by F. Moniliforme. And has 28 derivatives. FB is the most universal one of the derivatives, which mainly contaminate corn and their products. FB has a toxin effects to animals because of its powerful toxicity. As study shown that FB can cause the Leukoencephalopathy softening syndrome of horses and Pulmonary edema syndrome of pigs and can also induce human esophageal cancer, liver cancer, gastric cancer, and other diseases, so it poses a threat to husbandry and human health.
This kit is toxin detection products from the latest ELISA technology research and development, it has advantages of rapid, simple, accurate and high sensitivity compared with the instrument analysis technology, and its operation time only 15 min and can also minimize the work intensity and operation error.
| Manufacturer | Meizheng |
| Trademark | ToxinFast® |
| Order No. | HEM1296 |
| Analyte | Fumonisins |
| Sample Type | Feed,Corn, and Corn by- products. |
| Methodology | Indirect competitive enzyme-labeled immunoassay. |
| Packaging | 96 Wells |
| Storage | The kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE! |
| Shelf life | The validity for this kit is 12 months |
| Lead time | Shipped within 5-12 working days. |
| Cross Reactivity | FB…………………………………………100% FB2…………………………………………71% FB3…………………………………………85% |
| Calculate Result | Analyze the result by using the software |
| Sensitivity | 5 ppb |
| Limit of detection | 250 ppb |
| Percent Recovery, % | 100±20% |
| Precision (CV %): | Intra-lab assay: CV%≤10% |
Provided Materials and Reagent:
| Component | Specs |
| Antigen coated plate | 96 wells |
| 5 vials of standard * | 1mL each |
| FB Enzyme conjugate | 12mL |
| FB Antibody Solution | 12mL |
| Substrate | 10mL×2 |
| Concentrated Wash Buffer (10×) | 40mL |
| Package Insert | 1 |
| Test report | 1 |
| Plate cover | 2 |
Note: The concentration of the standards is 0µg/kg, 5µg/kg, 15µg/kg, 45µg/kg, 135µg/kg.
Materials Required But Not Provided
- Devices:
—- Microwell plate reader: 450nm/630nm
—- Vortex
—- Centrifuge (or funnel)
—- Balance: sensibility: 0.01g
—- Graduate Pipette: 10mL
—- Aurilave
—- Graduate cylinder: 50mL
—- Conical flask with stopper: 100mL
—- Centrifuge tube: 7mL
—- Quantitative analysis filter paper
—- Micropipette: Single channel pipet (10µL-100µL, 100µL-1000µL)
Multi-channel pipet (30µL-300µL)
- Reagent:
—- Methanol (AR)
—- Deionized water
—- 18M concentrated sulfuric acid (H2SO4)
Note: The pictures shown are sample product pictures.
For the actual product, please check Provided Materials and Reagent or consult the sales manager.
