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Food and Feed Antibiotic Chloramphenicol ELISA Test Kit

Qualitative or quantitative analysis of Chloramphenicol in animal tissue (including aquatic product tissue), milk, milk powder, honey, royal jelly, feed, swine urine, etc.

Order No.: SC0111

【Introduction】 

Chloramphenicol is a broad-spectrum antibiotic which is frequently employed in animal production for its excellent antibacterial and pharmacokinetic properties. However, in humans it leads to hematotoxic side effects, in particular chloramphenicol-induced aplastic anaemia for which a dosage-effect relationship has not yet been established. This has led to a prohibition of chloramphenicol for the treatment of animals used for food production.

【Principle of Test】 

Chloramphenicol ELISA Test Kit is an indirect competitive enzyme-labeled immunoassay. The chloramphenicol antigen is precoated on the wells. The precoated antigen compete the chloramphenicol antibody (antibody solution) with sample chloramphenicol in the, anti-chloramphenicol antibody binds to the chloramphenicol-HRP enzyme conjugate. Then pipet the substrate solutions to the wells to convert the color.

The color of unknown samples is compared to the color of the standards and the chloramphenicol concentration of the samples is derived.

Cross-reactivity

Chloramphenicol……………………………………………100%

Chloramphenicol Base……………………………………………0.5%

Methyl Chloramphenicol………………………………………≤0.05%

Florfenicol……………………………………………………… <0.01%

Florfenicol Amine………………………………………………..<0.1%

Materials Required But Not Provided

  1.  Devices:

—- Microwell Plate Reader (450nm/630nm)

—- Vortex

—- Centrifuge

—- Constant Temperature Incubator / Water bath

—- Termovap Sample Concentrator

—- Balance (sensibility: 0.01g)

—- Rubber suction bulb

—- Graduated pipette: 10mL

—- Centrifuge tube: 7mL / 10mL, 50mL

—- Micropipette: Single channel pipet (10µL-100µL, 100µL-1000µL)

Multi-channel pipet (30-300µL)

  1. Reagent:

—- Deionized water

—- Ethyl acetate (AR)

—- N-hexane (AR)

—- Sodium carbonate (Na2CO3) (AR)

—- Sodium hydrogen carbonate (NaHCO3) (AR)

—- 18M concentrated sulfuric acid (H2SO4)

Provided Materials and Reagent

Component96 wells
Antigen coated plate12 × 8 wells
6 vials of standard1.5mL each
High Concentration Standard (100ppb)1.5mL
Enzyme Conjugate6mL
Antibody Solution4mL
Substrate A6mL
Substrate B6mL
Concentrated Wash Buffer (10×)40mL
Concentrated Sample Diluting Buffer40mL
Concentrated Milk Powder Sample Extraction Buffer40mL
Package Insert1
Plate cover sheet2
Testing Report1

Note: The concentrations of the standards are 0μg/kg, 0.01μg/kg, 0.03μg/kg, 0.09μg/kg, 0.27μg/kg and 0.81μg/kg.

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