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Food Antibiotic Chloramphenicol ELISA Test Kit for Dairy, Honey and Feed
Qualitative or quantitative analysis of Chloramphenicol in animal tissue (including aquatic product tissue), milk, milk powder, honey, royal jelly, feed, swine urine, etc.
Background:
Chloramphenicol is a broad spectrum antibiotic which is frequently employed in animal production for its excellent antibacterial and pharmacokinetic properties. However, in humans it leads to hematotoxic side effects, in particular chloramphenicol-induced aplastic anaemia for which a dosage-effect relationship has not yet been established. This has led to a prohibition of chloramphenicol for the treatment of animals used for food production.
Manufacturer | Meizheng |
Order No. | SC0111 |
Analyte | Chloramphenicol |
Sample Type | Animal tissue (including aquatic product tissue), milk, milk powder, honey, royal jelly, feed, swine urine, etc.
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Methodology | Indirect competitive enzyme-labeled immunoassay. |
Packaging | 96 Wells |
Storage | The kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE! |
Shelf life | The validity for this kit is 12 months |
Lead time | Shipped within 5-12 working days. |
Cross Reactivity | Chloramphenicol……………………………100% Chloramphenicol Base…………………..…0.5% Methyl Chloramphenicol…………………………≤0.05% Florfenicol………………………………..<0.01% Florfenicol Amine………………………………………<0.1% |
Calculate Result | Analyze the result by using the software |
Sensitivity | 0.01ppb |
Limit of detection | Tissue, dairy, milk powder, honey, royal jelly……………………………………………….0.02ppb Raw milk, feed………………………..…0.04ppb Swine urine………………………………0.1ppb |
Percent Recovery, % | 80±10% |
Precision (CV %): | Intra-lab assay: CV<10% |
Provided Materials and Reagent
Component | 96 wells |
Antigen coated plate | 96T |
6 vials of standard | 1.5mL each |
High Concentration Standard (100ppb) | 1.5mL |
Enzyme Conjugate | 6mL |
Antibody Solution | 4mL |
Substrate A | 6mL |
Substrate B | 6mL |
Concentrated Wash Buffer (10×) | 40mL |
Concentrated Sample Diluting Buffer | 40mL |
Concentrated Milk Powder Sample Extracting Buffer | 40ml |
Package Insert | 1 |
Plate cover sheet | 2 |
Testing Report | 1 |
Note: The concentrations of the standards are 0ppb, 0.01ppb, 0.03ppb, 0.09ppb, 0.27ppb and 0.81ppb.
Materials Required But Not Provided
1 . Devices:
—- Microwell plate Reader (450nm/630nm)
—- Vortex mixer
—- Centrifuge
—- Thermostat incubator
—- Balance (sensibility: 0.01g)
—- Nitrogen evaporator/Rotatory evaporator
—- Water bath
—- Graduated pipette: 10mL
—- Micropipette: Single channel pipet (10µL-100µL, 100µL-1000µL)
Multi-channel pipet (30-300µL)
- Reagent:
—- Ethyl acetate (AR)
—- Deionized water
—- N-hexane (AR)
—- Na2CO3 (AR)
—- NaHCO3 (AR)
—- Concentrated sulfuric acid (18 mol/L)
Note: The pictures shown are sample product pictures.
For the actual product, please check Provided Materials and Reagent or consult the sales manager.
Quantity | 1-10, 11-100, 101-1000, 1001-10000 |
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