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Food Mycotoxin Deoxynivalenol (DON) ELISA Test Kit

The Deoxynivalenol ELISA Kit is a competitive ELISA for the qualitative or quantitative analysis of Deoxynivalenol in wheat, wheat flour, bran and wheat middlings, etc.

Brochure-Grains & Feed Test. PDF

Meizheng Bio-Tech Company Overview. PDF

Background:

Deoxynivalenol is known as vomitoxin or DON. It is a potent fungal toxin to affect crops grown in cool and moist conditions. Fusarium graminearum is the principal DON-producing fungus in grains but Fusarium culmorum is often involved as well especially in certain geographical areas of the world. Deoxynivalenol is a known immunosuppressant and may cause kidney problems. Consuming DON-contaminated grain can cause vomiting in humans and animals. Deoxynivalenol is formed by the Fusarium species and mainly affects corn, wheat, oats and barley.

ManufacturerMeizheng
TrademarkToxinFast®
Order No.HEM1896-2
AnalyteDeoxynivalenol
Sample TypeWheat, Wheat Flour, Bran And Wheat Middlings, etc.
MethodologyIndirect competitive enzyme-labeled immunoassay.
Packaging96 Wells
StorageThe kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE!
Shelf lifeThe validity for this kit is 12 months
Lead timeShipped within 5-12 working days.
Cross ReactivityDON…………………………………………100%
3-AcDON……………………………………<70%
15-AcDON……………..……………………<1%
Oxynivalenol ………………………………<1%
 Calculate ResultAnalyze the result by using the software
Limit of quantitation250 ppb
Limit of detection200 ppb
Percent Recovery, %Rice flour and milkl: 100±25%
Precision (CV %):Intra-lab assay: CV%≤10%

Provided Materials and Reagent:

Component96wells
Antigen coated plate12 X 8 wells
5 vials of Standard1mL each
DON Enzyme Conjugate12mL
DON Antibody Solution12mL
Substrate10mL*2
Stop Solution6mL*2
Concentrated Wash Solution (10X)40mL
Package Insert1
Plate Cover Sheet2
Testing Report1

 Note: The concentrations of the standards are 0µg/kg, 200µg/kg, 700µg/kg, 2100µg/kg and 7000µg/kg respectively (the real concentration of the standards is 1/25 of the set concentrations).

Materials Required But Not Provided

Devices:

—- Microwell plate Reader (450nm/630nm)

—- Oscillator/ Vortex

—- Thermostat incubator ( If Room temperature can reach 25℃, it is not needed.)

—- Centrifuge (or quantitative filter)

—- Balance (sensibility: 0.01g)

—- Pipette: 50mL

—- Triangular flask (with stopper): 100mL

—- Centrifuge tube: 7mL

—- Micropipet: Single channel pipet (10µL-100µL, 100µL-1000µL)  Multi-channel pipet (30µL-300µL)

Reagent:

—- Deionized water

Note: The pictures shown are sample product pictures.

For the actual product, please check Provided Materials and Reagent or consult the sales manager.

Quantity

1-10, 11-100, 101-1000, 1001-10000

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