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    Food Veterinary Drugs Residues Furazolidone ELISA Test Kit

    The Furazolidone (AOZ) ELISA Kit is competitive ELISA for the qualitative or quantitative analysis of Furazolidone (AOZ) in samples of poultry tissues, liver, eggs etc.

    Meizheng Bio-Tech Company Overview. PDF

    Background:

    Chloramphenicol (CAP) is a widely used antibiotic, but has serious side effects, which can cause aplastic anemia, agranulocytosis and other diseases.

    The detection method is sensitive, fast, simple and specific, only need 45minu for operating and a small amount of sampling.

    Use Principles: 

    The Chloramphenicol ELISA kit is an indirect competitive enzyme-labeled immunoassay. The antigen is precoated on the wells. The precoated antigen compete the antibody (antibody solution) with Chloramphenicol in the sample, anti-Chloramphenicol antibody binds to the HRP enzyme conjugate. Then pipe the substrate solution to the wells to convert the color.

    The color of unknown samples is compared to the color of the standards and the Chloramphenicol concentration of the samples is derived.

    ManufacturerMeizheng
    Order No.ME03A
    AnalyteFurazolidone
    Sample TypePoultry tissues, liver, eggs etc.
    MethodologyIndirect competitive enzyme-labeled immunoassay.
    Packaging96 Wells
    StorageThe kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE!
    Shelf lifeThe validity for this kit is 12 months
    Lead timeShipped within 5-12 working days.
    Cross ReactivityFurazolidone (AOZ)………………………100%
    Furantoin (AHD)…………………………<0.1%
    Furaltadone (AMOZ)……………………<0.1%
    Furacilin (SEM)…………………………<0.1%
     Calculate ResultAnalyze the result by using the software
    Sensitivity 0.03ppb
    Limit of detection0.1ppb
    Percent Recovery, %Tissues, liver, egg………………………100±30%
    Precision (CV %):Intra-lab assay: CV%≤10%

    Provided Materials and Reagent

    Component96 wells
    Antigen coated plate12 test strips of 8 wells each
    6 vials of standard1mL each
    High concentrated standard (100ppb)1mL
    AOZ Enzyme Conjugate6mL
    AOZ Antibody Solution6mL
    Substrate A6mL
    Substrate B6mL
    Concentrated wash buffer (10×)40mL
    Concentrated sample diluting buffer (2X)40mL
    Concentrated derivatization reagent (10X)1mL
    IFU1
    Testing Report1

    Note: The concentrations of the standards are 0ppb, 0.03ppb, 0.09ppb, 0.27ppb, 0.81ppb and 2.43ppb.

    Materials Required But Not Provided

    1. Devices:

    ---- Microwell plate Reader (450nm/630nm)

    ---- Vortex mixer

    ---- Centrifuge

    ---- Homogenizer

    ---- Constant temperature incubator

    ---- Balance (sensibility: 0.01g)

    ---- Nitrogen evaporator

    ---- Water bath

    ---- Transfer pipette: 10mL

    ---- Centrifuge tube: 10mL, 50mL,7mL

    ---- Micropipette: Single channel pipette (10µL-100µL, 100µL-1000µL)

    Multi-channel pipette (30-300µL)

    1. Reagent:

    ---- Ethyl acetate (AR)

    ---- Deionized water

    ---- N-hexane (AR)

    ---- Methanol (AR)

    ---- Concentrated hydrochloric acid (AR)

    ---- NaOH (AR)

    ---- Dipotassium Hydrogen Phosphate Trihydrate (K2HPO4.3H2O) (AR)

    ---- Concentrated sulfuric acid (18 mol/L)

    Note: The pictures shown are sample product pictures.

    For the actual product, please check Provided Materials and Reagent or consult the sales manager.

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