Background:
Nitrofurans, which are effective against a wide range of bacteria, include furazolidone, nitrofurazone, nitrofurantoin and related compounds. The food and drug administration has prohibited all uses of nitrofuran drugs in food-producing animals because they pose a public health risk. Evidence indicates that the drugs may induce carcinogenic residues in animal tissues.
Use Principles：
Furacilin Metabolite (SEM) ELISA Test Kit is an indirect competitive enzyme-labeled immunoassay. The SEM antigen is precoated on the wells. The precoated antigen compete the SEM antibody (antibody solution) with SEM in the sample, anti-SEM antibody binds to the SEM-HRP enzyme conjugate. Then pipet the substrate solutions to the wells to convert the color.
The color of unknown samples is compared to the color of the standards and the SEM concentration of the samples is derived.
| Manufacturer | Meizheng |
| Order No. | SC0041 |
| Analyte | Furacilin Metabolite |
| Sample Type | Honey, raw milk, etc. |
| Methodology | Indirect competitive enzyme-labeled immunoassay. |
| Packaging | 96 Wells |
| Storage | The kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE! |
| Shelf life | The validity for this kit is 12 months |
| Lead time | Shipped within 5-12 working days. |
| Cross Reactivity | Furacilin metabolite (SEM) 100% Furaltadone metabolite (AMOZ) <0.1% Furazolidone metabolite (AOZ) <0.1% Furantoin metabolite (AHD) <0.1% |
| Calculate Result | Analyze the result by using the software |
| Sensitivity | 0.05ppb |
| Limit of detection | 0.1ppb |
| Percent Recovery, % | 100±20% |
| Precision (CV %): | Intra-lab assay: CV<10% |
Provided Materials and Reagent
| Component | 96 T |
| Antigen coated plate | 96 wells |
| 6 vials of standard | 1mL each |
| High Concentration Standard (100ppb) | 1mL |
| Enzyme conjugate | 6mL |
| Antibody solution | 6mL |
| Substrate A | 6mL |
| Substrate B | 6mL |
| Concentrated wash buffer (10×) | 40mL |
| Concentrated sample diluting buffer (2×) | 40mL |
| Concentrated derivatization reagent (10×) | 1mL |
| IFU | 1 |
| Testing report | 1 |
| Plate cover sheet | 2 |
Note: The concentrations of the standards are 0ppb, 0.05ppb, 0.15ppb, 0.45ppb, 1.35ppb and 4.05ppb.
Materials Required But Not Provided
- Devices:
—- Microwell plate reader (450nm/630nm)
—- Vortex
—- Centrifuge
—- Incubator
—- Water bath
—- Nitrogen evaporator
—- Balance (sensibility: 0.01g)
—- Rubber bulb
—- Transfer pipette: 10mL
—- Centrifuge tube: 10mL/7mL, 50mL
—- Micropipette: Single channel pipette (10µL-100µL, 100µL-1000µL)
Multi-channel pipette (30-300µL)
- Reagent:
—- Deionized water
—- Ethyl acetate (AR)
—- N-hexane (AR)
—- Methanol (AR)
—- Sodium hydroxide (AR)
—- Concentrated hydrochloric acid (AR)
—- Dipotassium Hydrogen Phosphate Trihydrate (K2HPO4.3H2O) (AR)
—- Concentrated sulfuric acid (18mol/L)
Note: The pictures shown are sample product pictures.
For the actual product, please check Provided Materials and Reagent or consult the sales manager.
