Furazolidone Metabolite ELISA Test Kit for Poultry Tissues, Liver, Eggs and Egg Powder

【Summary】

Nitrofurans, which are effective against a wide range of bacteria, include furazolidone, nitrofurazone, nitrofurantoin and related compounds. The food and drug administration has prohibited all uses of nitrofuran drugs in food-producing animals because they pose a public health risk. Evidence indicates that the drugs may induce carcinogenic residues in animal tissues.

【Use Principles】

Furazolidone Metabolite ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The Furazolidone Metabolite antigen is precoated on the wells. The precoated antigen compete the Furazolidone Metabolite antibody (Antibody Solution) with Furazolidone Metabolite in the sample, Furazolidone Metabolite antibody binds to the Furazolidone Metabolite Enzyme Conjugate. After the color development of TMB Substrate, the absorbance value of the sample is negatively correlated with the Furazolidone Metabolite contained in it. Compared with the standard curve and multiplied by its corresponding dilution factor, the content of Furazolidone Metabolite in the sample can be calculated.

【Scope of Application】

Qualitative or quantitative analysis of Furazolidone Metabolite in samples of poultry tissues, liver, eggs, and egg powder etc.

【Cross-reactivity】

Furazolidone Metabolite 100%

Furaltadone Metabolite <0.1%

Furantoin Metabolite <0.1%

Furacilin Metabolite <0.1%

【Materials Required But Not Provided】

1. Devices:

—- Microplate Reader (450nm/630nm filter)

—- Vortex

—- Centrifuge

—- Homogenizer

—- Constant Temperature Incubator

—- Balance (sensibility: 0.01g)

—- Termovap Sample Concentrator

—- Water Bath

—- Graduated Pipette: 10mL

—- Rubber Bulb Syringe

—- Centrifuge Tube: 10mL, 50mL,7mL

—- Micropipette: Single channel pipette (10-100µL, 100-1000µL) Multi-channel pipette (30-300µL)

2. Reagent:

—- Ethyl acetate (AR)

—- Deionized water

—- N-hexane (AR)

—- Methanol (AR)

—- Concentrated hydrochloric acid (HCl) (AR)

—- Sodium hydroxide (NaOH) (AR)

—- Dipotassium Hydrogen Phosphate Trihydrate (K₂HPO₄‧3H₂O) (AR)

—- 18M concentrated sulfuric acid (H₂SO₄)

【Provided Materials and Reagent】

Note: The concentrations of the standards are 0ppb, 0.03ppb, 0.09ppb, 0.27ppb, 0.81ppb and 2.43ppb.