Ochratoxin A (OTA) is a toxic metabolite produced by Aspergillus flavus and Penicillium genera. Ochratoxin A has a strong liver and kidney toxicity, and has teratogenic, mutagenic, and carcinogenic.
OTA is the second important and harmful mycotoxin in the family following with Aflatoxin flavus. As the OTA producing fungi exist widely in nature, especially higher yield in cereals and cereals products, coffee, cocoa contaminated with OTA, it has been potential threat for human health and development of animal husbandry.
|Sample Type||Grain, Feed, etc.|
|Methodology||Indirect competitive enzyme-labeled immunoassay.|
|Storage||The kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE!|
|Shelf life||The validity for this kit is 12 months|
|Lead time||Shipped within 5-12 working days.|
|Cross Reactivity||Ochratoxin A………………………………100%|
|Calculate Result||Analyze the result by using the software|
|Limit of detection||2.5ppb, 1.25ppb,|
|Percent Recovery, %||100±20%|
|Precision (CV %):||Intra-lab assay: CV%≤10%|
Provided Materials and Reagent:
|Antigen coated plate||96 wells|
|5 vials of standard||1mL each|
|OTA Enzyme conjugate||12mL|
|OTA Antibody Solution||6mL|
|Concentrated sample diluting buffer||40mL|
|Concentrated Wash Buffer (10×)||40mL|
Note: The concentration of the standards is 0μg/kg, 0.05μg/kg, 0.15μg/kg, 0.45μg/kg, 1.35μg/kg.
Materials Required But Not Provided
---- Microwell plate Reader (450nm/630nm)
---- Vortex (or oscillator)
---- Balance (sensibility: 0.01g)
---- Transfer pipette: 10mL
---- Centrifuge tube: 50mL
---- Micropipette: Single channel pipet (10µL-100µL, 100µL-1000µL)
Multi-channel pipet (30µL-300µL)
---- Methanol (AR)
---- Deionized water
---- Concentrated sulfuric acid (18 mol/L)
Note: The pictures shown are sample product pictures.
For the actual product, please check Provided Materials and Reagent or consult the sales manager.