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Grains and Feed Ochratoxin A ELISA Test Kit

The Ochratoxin A ELISA Kit is an indirect competitive ELISA for the quantitative analysis of Ochratoxin A in grain, feed, etc.

Brochure-Grains & Feed Test. PDF

Meizheng Bio-Tech Company Overview. PDF

Background:

Ochratoxin A (OTA) is a toxic metabolite produced by Aspergillus flavus and Penicillium genera. Ochratoxin A has a strong liver and kidney toxicity, and has teratogenic, mutagenic, and carcinogenic.

OTA is the second important and harmful mycotoxin in the family following with Aflatoxin flavus. As the OTA producing fungi exist widely in nature, especially higher yield in cereals and cereals products, coffee, cocoa contaminated with OTA, it has been potential threat for human health and development of animal husbandry.

ManufacturerMeizheng
TrademarkToxinFast®
Order No.HEM1096
AnalyteOchratoxin A
Sample TypeGrain, Feed, etc.
MethodologyIndirect competitive enzyme-labeled immunoassay.
Packaging96 Wells
StorageThe kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE!
Shelf lifeThe validity for this kit is 12 months
Lead timeShipped within 5-12 working days.
Cross ReactivityOchratoxin A………………………………100%
 Calculate ResultAnalyze the result by using the software
Sensitivity 0.05 ppb
Limit of detection2.5ppb, 1.25ppb,
Percent Recovery, %100±20%
Precision (CV %):Intra-lab assay: CV%≤10%

Provided Materials and Reagent:

ComponentSpecs
Antigen coated plate96 wells
5 vials of standard1mL each
OTA Enzyme conjugate12mL
OTA Antibody Solution6mL
Substrate A6mL
Substrate B6mL
Concentrated sample diluting buffer40mL
Concentrated Wash Buffer (10×)40mL
Package Insert1
Testing Report1
Plate cover2

Note: The concentration of the standards is 0μg/kg, 0.05μg/kg, 0.15μg/kg, 0.45μg/kg, 1.35μg/kg.

Materials Required But Not Provided

  1. Devices:

—- Microwell plate Reader (450nm/630nm)

—- Vortex (or oscillator)

—- Centrifuge

—- Balance (sensibility: 0.01g)

—- Transfer pipette: 10mL

—- Aurilave

—- Centrifuge tube: 50mL

—- Micropipette: Single channel pipet (10µL-100µL, 100µL-1000µL)

Multi-channel pipet (30µL-300µL)

  1. Reagent:

—- Methanol (AR)

—- Deionized water

—- Concentrated sulfuric acid (18 mol/L)

Note: The pictures shown are sample product pictures.

For the actual product, please check Provided Materials and Reagent or consult the sales manager.

Quantity

1-10, 11-100, 101-1000, 1001-10000

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