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    Grains and Wheat Mycotoxin Zearalenone (ZEN) ELISA Test Kit

    The Zearalenone ELISA Kit is a competitive ELISA for the qualitative or quantitative analysis of zearalenone in edible oil, corn, wheat, flour, barley, oat, etc.

    Brochure-Grains & Feed Test. PDF

    Meizheng Bio-Tech Company Overview. PDF

    Background:

    The mycotoxin zearalenone is formed by fungi of the genus fusarium and mainly affects corn, wheat, sorghum, barley and rye. Zearalenone is a phytohormone which displays, apart from its anabolic properties, mainly estrogenic effects. Because of its estrogenic properties, zearalenone may induce fertility disorders in animals with clinical signs of hyperestrogenism – an aspect of a disease which although reported mainly in hogs, is described in other species such as cow, horse and sheep.

    The potential health risk for man induced by this mycotoxin, which is taken up with foods of vegetable or animal origin, is extensively discussed. Such fusarium species are known to co-produce other toxins such as deoxynivalenol.

    ManufacturerMeizheng
    TrademarkToxinFast®
    Order No.HEM0696
    AnalyteZearalenone
    Sample TypeCorn, Wheat, Flour, Barley, Oat, Etc.
    MethodologyIndirect competitive enzyme-labeled immunoassay.
    Packaging96 Wells
    StorageThe kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE!
    Shelf lifeThe validity for this kit is 12 months
    Lead timeShipped within 5-12 working days.
    Cross ReactivityZearalenone……………100%
    Zearalanone……………13%
    Zeranol…………………<1%
    Result InterpretationAnalyze the result by using the software
    Sensitivity0.2ppb
    Limit of detection20ppb
    Percent Recovery  100±20%
    Intra-lab assayCV%≤10%

    Provided Materials and Reagent:

    Component96 wells
    Antigen Coated Plate96T
    5 Vials of Standard1mL each
    ZEN Enzyme Conjugate6mL
    ZEN Antibody Solution6mL
    Substrate A6mL
    Substrate B6mL
    Stop Solution6mL
    ZEN Concentrated Sample Diluting Solution40mL
    Concentrated Wash Solution (10X)40mL
    Package Insert1
    Plate Cover Sheet2
    Testing Report1

    Note: The concentrations of the standard are 0ppb, 0.2ppb, 0.6ppb, 2.5ppb and 7.5ppb.

    Materials Required But Not Provided

    Devices:

    ---- Microwell plate reader (450nm/630nm)

    ---- Oscillator/ Vortex

    ---- Centrifuge

    ---- Balance (sensibility: 0.01g)

    ---- Graduated pipette: 10mL

    ---- Aurilave

    ---- Funnel

    ---- Quantitative filter

    ---- Centrifuge tube: 50mL

    ---- Micropipette: Single channel pipet (10µl-100µl,  100µl-1000µl)

    Multi-channel pipet (30µl-300µl)

    Reagent:

    ---- Methanol (AR)

    ---- Deionized water

    Note: The pictures shown are sample product pictures.

    For the actual product, please check Provided Materials and Reagent or consult the sales manager.

    Quantity

    1-10, 11-100, 101-1000, 1001-10000

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