Background:
The mycotoxin zearalenone is formed by fungi of the genus fusarium and mainly affects corn, wheat, sorghum, barley and rye. Zearalenone is a phytohormone which displays, apart from its anabolic properties, mainly estrogenic effects. Because of its estrogenic properties, zearalenone may induce fertility disorders in animals with clinical signs of hyperestrogenism – an aspect of a disease which although reported mainly in hogs, is described in other species such as cow, horse and sheep.
The potential health risk for man induced by this mycotoxin, which is taken up with foods of vegetable or animal origin, is extensively discussed. Such fusarium species are known to co-produce other toxins such as deoxynivalenol.
| Manufacturer | Meizheng |
| Trademark | ToxinFast® |
| Order No. | HEM0696 |
| Analyte | Zearalenone |
| Sample Type | Corn, Wheat, Flour, Barley, Oat, Etc. |
| Methodology | Indirect competitive enzyme-labeled immunoassay. |
| Packaging | 96 Wells |
| Storage | The kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE! |
| Shelf life | The validity for this kit is 12 months |
| Lead time | Shipped within 5-12 working days. |
| Cross Reactivity | Zearalenone……………100% Zearalanone……………13% Zeranol…………………<1% |
| Result Interpretation | Analyze the result by using the software |
| Sensitivity | 0.2ppb |
| Limit of detection | 20ppb |
| Percent Recovery | 100±20% |
| Intra-lab assay | CV%≤10% |
Provided Materials and Reagent:
| Component | 96 wells |
| Antigen Coated Plate | 96T |
| 5 Vials of Standard | 1mL each |
| ZEN Enzyme Conjugate | 6mL |
| ZEN Antibody Solution | 6mL |
| Substrate A | 6mL |
| Substrate B | 6mL |
| Stop Solution | 6mL |
| ZEN Concentrated Sample Diluting Solution | 40mL |
| Concentrated Wash Solution (10X) | 40mL |
| Package Insert | 1 |
| Plate Cover Sheet | 2 |
| Testing Report | 1 |
Note: The concentrations of the standard are 0ppb, 0.2ppb, 0.6ppb, 2.5ppb and 7.5ppb.
Materials Required But Not Provided
Devices:
—- Microwell plate reader (450nm/630nm)
—- Oscillator/ Vortex
—- Centrifuge
—- Balance (sensibility: 0.01g)
—- Graduated pipette: 10mL
—- Aurilave
—- Funnel
—- Quantitative filter
—- Centrifuge tube: 50mL
—- Micropipette: Single channel pipet (10µl-100µl, 100µl-1000µl)
Multi-channel pipet (30µl-300µl)
Reagent:
—- Methanol (AR)
—- Deionized water
Note: The pictures shown are sample product pictures.
For the actual product, please check Provided Materials and Reagent or consult the sales manager.
