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Grains and Wheat Mycotoxin Zearalenone (ZEN) ELISA Test Kit

The Zearalenone ELISA Kit is a competitive ELISA for the qualitative or quantitative analysis of zearalenone in edible oil, corn, wheat, flour, barley, oat, etc.

Brochure-Grains & Feed Test. PDF

Meizheng Bio-Tech Company Overview. PDF

Background:

The mycotoxin zearalenone is formed by fungi of the genus fusarium and mainly affects corn, wheat, sorghum, barley and rye. Zearalenone is a phytohormone which displays, apart from its anabolic properties, mainly estrogenic effects. Because of its estrogenic properties, zearalenone may induce fertility disorders in animals with clinical signs of hyperestrogenism – an aspect of a disease which although reported mainly in hogs, is described in other species such as cow, horse and sheep.

The potential health risk for man induced by this mycotoxin, which is taken up with foods of vegetable or animal origin, is extensively discussed. Such fusarium species are known to co-produce other toxins such as deoxynivalenol.

ManufacturerMeizheng
TrademarkToxinFast®
Order No.HEM0696
AnalyteZearalenone
Sample TypeCorn, Wheat, Flour, Barley, Oat, Etc.
MethodologyIndirect competitive enzyme-labeled immunoassay.
Packaging96 Wells
StorageThe kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE!
Shelf lifeThe validity for this kit is 12 months
Lead timeShipped within 5-12 working days.
Cross ReactivityZearalenone……………100%
Zearalanone……………13%
Zeranol…………………<1%
Result InterpretationAnalyze the result by using the software
Sensitivity0.2ppb
Limit of detection20ppb
Percent Recovery  100±20%
Intra-lab assayCV%≤10%

Provided Materials and Reagent:

Component96 wells
Antigen Coated Plate96T
5 Vials of Standard1mL each
ZEN Enzyme Conjugate6mL
ZEN Antibody Solution6mL
Substrate A6mL
Substrate B6mL
Stop Solution6mL
ZEN Concentrated Sample Diluting Solution40mL
Concentrated Wash Solution (10X)40mL
Package Insert1
Plate Cover Sheet2
Testing Report1

Note: The concentrations of the standard are 0ppb, 0.2ppb, 0.6ppb, 2.5ppb and 7.5ppb.

Materials Required But Not Provided

Devices:

—- Microwell plate reader (450nm/630nm)

—- Oscillator/ Vortex

—- Centrifuge

—- Balance (sensibility: 0.01g)

—- Graduated pipette: 10mL

—- Aurilave

—- Funnel

—- Quantitative filter

—- Centrifuge tube: 50mL

—- Micropipette: Single channel pipet (10µl-100µl,  100µl-1000µl)

Multi-channel pipet (30µl-300µl)

Reagent:

—- Methanol (AR)

—- Deionized water

Note: The pictures shown are sample product pictures.

For the actual product, please check Provided Materials and Reagent or consult the sales manager.

Quantity

1-10, 11-100, 101-1000, 1001-10000

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