Product Description:
The Histamine Rapid Test Kit combines a rapid visual colorimetric strip test for the determination of histamine in fresh fish, other seafood, fishmeal, fish sauce and milk with a semi-quantitative result.
Histamine is a contaminant that can be found in improperly handled seafood. Quality tuna has histamine levels below 50 ppm. High levels of histamine in seafood can cause scombroid poisoning. The amount of histamine can be determined using ELISA or LC-MS. However, these traditional methods require very expensive equipment and time-consuming sample preparation procedures. Using the Histamine Rapid Test Kit method, histamine concentrations can be interpreted semi-quantitatively by visual comparison to a color reference card. The kit contains a histamine standard to verify assay performance.
The Histamine Rapid Test Kit uses a colorimetric enzymatic assay to rapidly detect histamine. The unique features of the kit are:
- Rapid, semi-quantitativevisual procedure that takes only 4 minutes
- Simpleaqueousextraction
- Highreproducibility
KIT CONTENTS, STORAGE, & SHELF LIFE
The Histamine Rapid Test Kit contains 24 tests. The kit must be used by the expiration date provided on the kit and component labels.
| Kit Contents | Quantity | Storage |
| Microtiter Plate | 1 x 96-well plate | 2–8°C |
| Test Strips | 24 strips | 2–8°C |
| Seafood/Milk Buffer Concentrate | 10 mL | 2–8°C |
| Fish Meal Buffer Concentrate | 2×25 mL | 2–8°C |
| Balance Buffer | 5 mL | 2–8°C |
| Color Mix | 0.75 mL | 2–8°C |
| Neutralization Mix | 0.75 mL | 2–8°C |
| Positive Control | 2 mL | 2–8°C |
Required Materials Not Provided With the Kit
- 50-mLplastic tubes
- 10, 20, 100 and1000 µL Pipettes
- Graduatedcylinder
- Deionizedor distilled water
- Whatman™ #1 FilterPaper(alternative step for Fish Meal samples)
- 2-mLmicrocentrifuge tubes
Required Equipment Not Provided With the Kit
- Centrifuge
- VortexMixer
- Balance
- TissueHomogenizeror Food Processor
Note: The pictures shown are sample product pictures. For the actual product, please check Provided Materials and Reagent or consult the sales manager.
