Search

In Stock

Honey Qualitative or Quantitative Furaltadone Metabolite ELISA Test Kit

Qualitative or quantitative analysis of furaltadone metabolite in honey.

Order No.: SC0044

Summary 

Nitrofurans, which are effective against a wide range of bacteria. The metabolites of nitrofuran drugs are furacilin metabolite (SEM), furaltadone metabolite (AMOZ), furazolidone metabolite (AOZ), and furantoin metabolite (AHD), which combined with proteins stably. Therefore, the detection of their metabolites is often used to reflect the residual status of nitrofuran drugs. The food and drug administration has prohibited all uses of nitrofuran drugs in food-producing animals because they pose a public health risk. Evidence indicates that the drugs may induce carcinogenic residues in animal tissues.

Use Principles 

Furaltadone Metabolite (AMOZ) ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The AMOZ antigen is precoated on the wells. The precoated antigen compete the AMOZ antibody (antibody solution) with AMOZ in the sample, anti-AMOZ antibody binds to the AMOZ-HRP enzyme conjugate. Then pipette the substrate solutions to the wells to convert the color.

The color of unknown samples is compared to the color of the standards and the AMOZ concentration of the samples is derived.

Cross-reactivity

Furaltadone metabolite (AMOZ)  100%

Furantoin metabolite (AHD) <0.1%

Furazolidone metabolite (AOZ) <0.1%

Furacilin metabolite (SEM) <0.1%

Materials Required but Not Provided

  1. Devices:

—- Microwell plate Reader (450nm/630nm)

—- Oscillator / Vortex

—- Centrifuge

—- Constant Temperature Incubator / Water bath

—- Balance (sensibility: 0.01g)

—- Termovap Sample Concentrator

—- Rubber suction bulb

—- Graduated pipette: 10mL

—- Centrifuge tube: 7mL / 10mL, 50mL

—- Micropipette: Single channel pipette (10-100μL, 100-1000μL)

Multi-channel pipette (30-300μL)

  1. Reagent:

—- Deionized/distilled water

—- Ethyl acetate (AR)

—- N-hexane (AR)

—- Methanol (AR)

—- Sodium hydroxide (NaOH) (AR)

—- Concentrated hydrochloric acid (HCl) (AR)

—- Dipotassium hydrogen phosphate trihydrate (K2HPO4·3H2O) (AR)

—- 18M concentrated sulfuric acid (H2SO4)

Provided Materials and Reagent

Component 96 wells
Antigen coated plate12 × 8 wells
6 vials of standard1mL each
AHD-AOZ-AMOZ-SEM Mixed High Concentration Standard (100ppb)1mL
Enzyme Conjugate6mL
Antibody Solution6mL
Substrate A6mL
Substrate B6mL
Concentrated Wash Buffer (10×)40mL
Concentrated Sample Diluting Buffer40mL
Concentrated Derivatization Reagent (10×)1mL
Package Insert1
Testing Report1
Plate cover sheet2

Note: The concentrations of the standards are 0μg/kg, 0.05μg/kg, 0.15μg/kg, 0.45μg/kg, 1.35μg/kg and 4.05μg/kg.

Bestsellers

Aflatoxin B1 Quantitative Rapid Lateral Flow Test Kit

The kit is a lateral flow immunochromatographic assay that determines a quantitative level for the presence of aflatoxin B1 and is intended for use in nuts, grain and feed. This method is suitable for the rapid on-site test.Brochure-Grains & Feed Test. PDFMeizheng Bio-Tech Company Overview. PDF

Aflatoxin M1 Immunoaffinity Column

Order No.: QC0068,QC0069,QC0131,QC0071Column Capacity:100 ngVolumn: 3mLPackage: 25/50T 

Immunoaffinity Column for Aflatoxin B1,B2,G1,G2

The immunoaffinity column can selectively adsorb aflatoxins (B1, B2, G1, G2) from the sample solution, thereby purifying the sample. The purified sample solution can then be directly used for HPLC or LC-MS/MS analysis.Affinity columns can be used in combination with HPLC or LC-MS/MS to achieve rapid testing, and to increase signal-noise ratio  and improve the accuracy of the detection method.

Immunoaffinity Column for 2-in-1 Aflatoxins (B1,B2,G1,G2) and Ochratoxin A

The immunoaffinity column can selectively adsorb aflatoxin (B1, B2, G1, G2) and Ochratoxin A from the sample solution, thereby having a highly targeted purification effect on the sample. The sample  solution that has been purified by passing through the affinity column can be directly used for HPLC analysis or for LC-MS/MS analysis after concentrating with nitrogen gas and resuspended.Order No.: HCM3725B/HCM3750BProduct Properties: AFT B1/B2/G1/G2 300ng, OTA 100ngSpecifications: 3mL,25T/50T

Neomycin/Paromomycin, Kanamycin, Gentamicin and Spectinomycin Milk Comb Rapid Test Kit

The Neomycin/Paromomycin, Kanamycin, Gentamicin and Spectinomycin Rapid Test Kit is a lateral flow assay that determines a qualitative level for the presence of neomycin/ paromomycin, kanamycin, gentamicin and spectinomycin antibiotic residues in raw cow’s milk.Brochure-Dairy Test. PDFMeizheng Bio-Tech Company Overview. PDF

Beta-lactams, Tetracyclines, Streptomycin and Chloramphenicol Rapid Test Kit

The Beta-lactams, Tetracyclines, Streptomycin and Chloramphenicol Rapid Test Kit, 4-in-1 Rapid Test Kit is a Lateral Flow assay that determines the residues for the presence of target antibiotic residues in raw milk.Brochure-Dairy Test. PDFMeizheng Bio-Tech Company Overview. PDF

Total Aflatoxins Quantitative Rapid Lateral Flow Test Kit

The kit is a lateral flow immunochromatographic assay that determines a quantitative level for the presence of aflatoxin and is intended for use in grain and feed. This method is suitable for the rapid on-site test.Brochure-Grains & Feed Test. PDFMeizheng Bio-Tech Company Overview. PDF

 

Back to Top
Product has been added to your cart