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    Illegal Additive Melamine ELISA Test Kit for Dariy and Feed

    The Melamine ELISA Test Kit is a competitive ELISA for the Qualitative or quantitative analysis the residues of Melamine in milk, milk powder, yoghourt, feed, etc.

    Brochure-Dairy Test. PDF

    Meizheng Bio-Tech Company Overview. PDF

    Background:

    Melamine is an organic base and a trimer of cyanamide, with a 1,3,5-triazine skeleton. Melamine is also a metabolite of cyromazine, a pesticide. It is formed in the body of mammals that have ingested cyromazine. It has been reported that cyromazine can also be converted to melamine in plants.

    Melamine combines with cyanuric acid and related compounds to form melamine cyanurate and related crystal structures, which have been implicated as contaminants or biomarkers in Chinese protein adulterations.

    Melamine is described as "Harmful if swallowed, inhaled or absorbed through the skin. Chronic exposure may cause cancer or reproductive damage. Eye, skin and respiratory irritant".

    Use Principles: 

    Melamine ELISA Test Kit is an indirect competitive enzyme-labeled immunoassay. The melamine antigen is precoated on the wells. The precoated antigen compete the Melamine antibody (antibody solution) with Melamine in the sample, anti-Melamine antibody binds to the Melamine-HRP enzyme conjugate. Then pipet the substrate solutions to the wells to convert the color.

    The color of unknown samples is compared to the color of the standards and the Melamine concentration of the samples is derived.

    ManufacturerMeizheng
    Order No.SC0067
    AnalyteMelamine
    Sample TypeMilk, milk powder, yoghourt, feed, etc.
    MethodologyIndirect competitive enzyme-labeled immunoassay.
    Packaging96 Wells
    StorageThe kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE!
    Shelf lifeThe validity for this kit is 12 months
    Lead timeShipped within 5-12 working days.
    Cross ReactivityMelamine……………………………………100%
     Calculate ResultAnalyze the result by using the software
    Sensitivity 0.01ppb
    Limit of detectionFeed…………………………………………50ppb
    Milk Powder………………………………30ppb
    Yoghourt………………………….…….…15ppb
    Finished pure milk, raw milk……………20ppb
    Percent Recovery, %Milk Powder, finished pure milk, raw milk, yoghurt…… 100±20%
    Feed……………………………………100±30%
    Precision (CV %):Intra-lab assay: CV%≤10%

    Provided Materials and Reagent

    Component96 wells
    Antigen coated plate12 X 8 wells
    6 vials of Standard1mL each
    High Concentrated Standard (10ppm)1mL
    Enzyme Conjugate6mL
    Antibody Solution6mL
    Substrate A6mL
    Substrate B6mL
    Concentrated Wash Buffer (10X)40mL
    Concentrated sample diluting buffer40mL
    Package insert1
    Testing Report1
    Plate Cover Sheet2

    Note: The concentrations of the standard are 0ppb, 1ppb, 3ppb, 9ppb, 27ppb and 81ppb.

    Materials Required But Not Provided

    1. Devices:

    ----Microwell plate Reader (450nm/630nm)

    ---- Vortex

    ---- Centrifuge

    ---- Balance (sensibility: 0.01g)

    ---- Transfer pipette: 10mL

    ---- Centrifuge tube: 10mL, 50mL

    ---- Aurilave

    ---- Micropipette: Single channel pipet (10µL-100µL, 100µL-1000µL)

    Multi-channel pipet (30-300µL)

    1. Reagent:

    ---- Deionized water

    ---- Methanol (AR)

    ---- NaOH (AR)---- Concentrated sulfuric acid (18mol/L)

     Note: The pictures shown are sample product pictures.

    For the actual product, please check Provided Materials and Reagent or consult the sales manager.

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