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Illegal Additive Melamine ELISA Test Kit for Dariy and Feed

The Melamine ELISA Test Kit is a competitive ELISA for the Qualitative or quantitative analysis the residues of Melamine in milk, milk powder, yoghourt, feed, etc.

Brochure-Dairy Test. PDF

Meizheng Bio-Tech Company Overview. PDF

Background:

Melamine is an organic base and a trimer of cyanamide, with a 1,3,5-triazine skeleton. Melamine is also a metabolite of cyromazine, a pesticide. It is formed in the body of mammals that have ingested cyromazine. It has been reported that cyromazine can also be converted to melamine in plants.

Melamine combines with cyanuric acid and related compounds to form melamine cyanurate and related crystal structures, which have been implicated as contaminants or biomarkers in Chinese protein adulterations.

Melamine is described as “Harmful if swallowed, inhaled or absorbed through the skin. Chronic exposure may cause cancer or reproductive damage. Eye, skin and respiratory irritant”.

Use Principles: 

Melamine ELISA Test Kit is an indirect competitive enzyme-labeled immunoassay. The melamine antigen is precoated on the wells. The precoated antigen compete the Melamine antibody (antibody solution) with Melamine in the sample, anti-Melamine antibody binds to the Melamine-HRP enzyme conjugate. Then pipet the substrate solutions to the wells to convert the color.

The color of unknown samples is compared to the color of the standards and the Melamine concentration of the samples is derived.

ManufacturerMeizheng
Order No.SC0067
AnalyteMelamine
Sample TypeMilk, milk powder, yoghourt, feed, etc.
MethodologyIndirect competitive enzyme-labeled immunoassay.
Packaging96 Wells
StorageThe kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE!
Shelf lifeThe validity for this kit is 12 months
Lead timeShipped within 5-12 working days.
Cross ReactivityMelamine……………………………………100%
 Calculate ResultAnalyze the result by using the software
Sensitivity 0.01ppb
Limit of detectionFeed…………………………………………50ppb
Milk Powder………………………………30ppb
Yoghourt………………………….…….…15ppb
Finished pure milk, raw milk……………20ppb
Percent Recovery, %Milk Powder, finished pure milk, raw milk, yoghurt…… 100±20%
Feed……………………………………100±30%
Precision (CV %):Intra-lab assay: CV%≤10%

Provided Materials and Reagent

Component96 wells
Antigen coated plate12 X 8 wells
6 vials of Standard1mL each
High Concentrated Standard (10ppm)1mL
Enzyme Conjugate6mL
Antibody Solution6mL
Substrate A6mL
Substrate B6mL
Concentrated Wash Buffer (10X)40mL
Concentrated sample diluting buffer40mL
Package insert1
Testing Report1
Plate Cover Sheet2

Note: The concentrations of the standard are 0ppb, 1ppb, 3ppb, 9ppb, 27ppb and 81ppb.

Materials Required But Not Provided

  1. Devices:

—-Microwell plate Reader (450nm/630nm)

—- Vortex

—- Centrifuge

—- Balance (sensibility: 0.01g)

—- Transfer pipette: 10mL

—- Centrifuge tube: 10mL, 50mL

—- Aurilave

—- Micropipette: Single channel pipet (10µL-100µL, 100µL-1000µL)

Multi-channel pipet (30-300µL)

  1. Reagent:

—- Deionized water

—- Methanol (AR)

—- NaOH (AR)—- Concentrated sulfuric acid (18mol/L)

 Note: The pictures shown are sample product pictures.

For the actual product, please check Provided Materials and Reagent or consult the sales manager.

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