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    Illegal Additives Crystal violet ELISA Test Kit for Fish, Shrimp and Water

    Summary 

    Residues of Crystal violet (CV), a veterinary drug illegally used for the treatment of aqua-cultured fish. The detection of illegal additive residues such as Crystal violet in seafood and water is of great concern for the aquaculture because of the potential interfering and adverse effects of illegal additive residues frequently found in seafood and water.

    Use Principles 

    Crystal violet ELISA Test Kit is an indirect competitive enzyme-labeled immunoassay. The Crystal violet antigen is precoated on the wells. The precoated antigen compete the Crystal violet antibody (antibody solution) with Crystal violet in the sample, anti- Crystal violet antibody binds to the malachite green-HRP enzyme conjugate. Then pipette the substrate solutions to the wells to convert the color.

    The color of unknown samples is compared to the color of the standards and the Crystal violet concentration of the samples is derived.

    Scope of Application

    Qualitative or quantitative analysis of crystal violet, leuco-crystal violet, malachite green and leuco-malachite green residues in seafood (fish, shrimp), water, etc.

    Cross-reactivity

    Crystal violet……………….……………..………………………100%

    Leuco-Crystal violet…………….………...……….…...…………100%

    Malachite green…………………………..………………………67%

    Leuco-malachite green (oxidized)…..……...……….…...…………67%

    Materials Required But Not Provided

    1. Devices:

    ---- Microplate Reader (450nm/630nm filter)

    ---- Homogenizer

    ---- Vortex

    ---- Centrifuge

    ---- Constant Temperature Incubator

    ---- Balance (sensibility: 0.01g)

    ---- Graduated pipette: 10mL

    ---- Termovap Sample Concentrator

    ---- Rubber Bulb Syringe

    ---- Centrifuge tube: 5mL/7mL, 50mL

    ---- Micropipette: Single channel pipette (10-100µL, 100-1000µL)

    Multi-channel pipette (30-300µL)

    1. Reagent:

    ---- Deionized water

    ---- Acetonitrile (AR)

    ---- Sodium hydroxide (NaOH) (AR)

    Provided Materials and Reagent

    Component 96 wells
    Antigen coated plate96T
    6 vials of standard1mL each
    High concentration standard (100ppb)1mL
    Antibody Solution6mL
    Enzyme Conjugate12mL
    Substrate A6mL
    Substrate B6mL
    Concentrated Wash Buffer (10×)40mL
    Sample diluting buffer ×2 vials45mL/vial
    Sample extractant A ×2 bags90g/bag
    Sample extractant B ×2 bags90g/bag
    Oxidizer6mL
    Stop solution6mL
    Package Insert1
    Testing Report1
    Plate Cover Sheet2

    Note:

    1. The concentrations of the standards are 0ppb, 0.02ppb, 0.06ppb, 0.18ppb, 0.54ppb, and 1.62ppb.
    2. Sample extractant A and B: After each test, please seal the zip lock bag and put it in the aluminum foil bag with desiccant for sealing again.

    Note: The pictures shown are sample product pictures.

    For the actual product, please check Provided Materials and Reagent or consult the sales manager.

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