Search

In Stock

Illegal Additives Crystal violet ELISA Test Kit for Fish, Shrimp and Water

Summary 

Residues of Crystal violet (CV), a veterinary drug illegally used for the treatment of aqua-cultured fish. The detection of illegal additive residues such as Crystal violet in seafood and water is of great concern for the aquaculture because of the potential interfering and adverse effects of illegal additive residues frequently found in seafood and water.

Use Principles 

Crystal violet ELISA Test Kit is an indirect competitive enzyme-labeled immunoassay. The Crystal violet antigen is precoated on the wells. The precoated antigen compete the Crystal violet antibody (antibody solution) with Crystal violet in the sample, anti- Crystal violet antibody binds to the malachite green-HRP enzyme conjugate. Then pipette the substrate solutions to the wells to convert the color.

The color of unknown samples is compared to the color of the standards and the Crystal violet concentration of the samples is derived.

Scope of Application

Qualitative or quantitative analysis of crystal violet, leuco-crystal violet, malachite green and leuco-malachite green residues in seafood (fish, shrimp), water, etc.

Cross-reactivity

Crystal violet……………….……………..………………………100%

Leuco-Crystal violet…………….………………….………………100%

Malachite green…………………………..………………………67%

Leuco-malachite green (oxidized)…..……………….………………67%

Materials Required But Not Provided

  1. Devices:

—- Microplate Reader (450nm/630nm filter)

—- Homogenizer

—- Vortex

—- Centrifuge

—- Constant Temperature Incubator

—- Balance (sensibility: 0.01g)

—- Graduated pipette: 10mL

—- Termovap Sample Concentrator

—- Rubber Bulb Syringe

—- Centrifuge tube: 5mL/7mL, 50mL

—- Micropipette: Single channel pipette (10-100µL, 100-1000µL)

Multi-channel pipette (30-300µL)

  1. Reagent:

—- Deionized water

—- Acetonitrile (AR)

—- Sodium hydroxide (NaOH) (AR)

Provided Materials and Reagent

Component 96 wells
Antigen coated plate96T
6 vials of standard1mL each
High concentration standard (100ppb)1mL
Antibody Solution6mL
Enzyme Conjugate12mL
Substrate A6mL
Substrate B6mL
Concentrated Wash Buffer (10×)40mL
Sample diluting buffer ×2 vials45mL/vial
Sample extractant A ×2 bags90g/bag
Sample extractant B ×2 bags90g/bag
Oxidizer6mL
Stop solution6mL
Package Insert1
Testing Report1
Plate Cover Sheet2

Note:

  1. The concentrations of the standards are 0ppb, 0.02ppb, 0.06ppb, 0.18ppb, 0.54ppb, and 1.62ppb.
  2. Sample extractant A and B: After each test, please seal the zip lock bag and put it in the aluminum foil bag with desiccant for sealing again.

Note: The pictures shown are sample product pictures.

For the actual product, please check Provided Materials and Reagent or consult the sales manager.

Bestsellers

Aflatoxin B1 Quantitative Rapid Lateral Flow Test Kit

The kit is a lateral flow immunochromatographic assay that determines a quantitative level for the presence of aflatoxin B1 and is intended for use in nuts, grain and feed. This method is suitable for the rapid on-site test.Brochure-Grains & Feed Test. PDFMeizheng Bio-Tech Company Overview. PDF

Neomycin/Paromomycin, Kanamycin, Gentamicin and Spectinomycin Milk Comb Rapid Test Kit

The Neomycin/Paromomycin, Kanamycin, Gentamicin and Spectinomycin Rapid Test Kit is a lateral flow assay that determines a qualitative level for the presence of neomycin/ paromomycin, kanamycin, gentamicin and spectinomycin antibiotic residues in raw cow’s milk.Brochure-Dairy Test. PDFMeizheng Bio-Tech Company Overview. PDF

Immunoaffinity Column for Aflatoxin B1,B2,G1,G2

The immunoaffinity column can selectively adsorb aflatoxins (B1, B2, G1, G2) from the sample solution, thereby purifying the sample. The purified sample solution can then be directly used for HPLC or LC-MS/MS analysis.Affinity columns can be used in combination with HPLC or LC-MS/MS to achieve rapid testing, and to increase signal-noise ratio  and improve the accuracy of the detection method.

Aflatoxin M1 Immunoaffinity Column

Order No.: QC0068,QC0069,QC0131,QC0071Column Capacity:100 ngVolumn: 3mLPackage: 25/50T 

Immunoaffinity Column for 2-in-1 Aflatoxins (B1,B2,G1,G2) and Ochratoxin A

The immunoaffinity column can selectively adsorb aflatoxin (B1, B2, G1, G2) and Ochratoxin A from the sample solution, thereby having a highly targeted purification effect on the sample. The sample  solution that has been purified by passing through the affinity column can be directly used for HPLC analysis or for LC-MS/MS analysis after concentrating with nitrogen gas and resuspended.Order No.: HCM3725B/HCM3750BProduct Properties: AFT B1/B2/G1/G2 300ng, OTA 100ngSpecifications: 3mL,25T/50T

Aflatoxin M1 Qualitative Lateral Flow Rapid Test Kit

Aflatoxin M1 Qualitative Rapid Test Kit is a lateral flow assay that determines a qualitative level for the presence of aflatoxin M1 in raw cow’s milk. This test is designed for rapid field use or daily control in laboratories.Brochure-Dairy Test. PDFMeizheng Bio-Tech Company Overview. PDF

Tetracyclines ELISA Test Kit for Animal Tissue, Seafood and Egg

Qualitative or quantitative analysis of tetracycline, oxytetracycline, aureomycin, doxycycline in animal tissue, seafood and egg, etc.Meizheng Bio-Tech Company Overview. PDF

 

Back to Top
Product has been added to your cart