Immunoaffinity Column 3-in-1 Zearalenone/Deoxynivalenol/Ochratoxin A

The immunoaffinity column can selectively adsorb Zearalenone (ZEN),Deoxynivalenol(DON) and Ochratoxin A (OTA) from the sample solution, thereby having a highly targeted purification effect on the sample. The sample solution that has been purified by passing through the affinity column can be directly used for HPLC or LC-MS/MS analysis after concentrating with nitrogen gas and resuspended.

Order No.: HCM6225B/HCM6250B

1. Principle:

The basis of the measurement is the antigen-antibody reaction. Antibodies are connected to the column and the toxins in the sample are extracted, filtered, and diluted, and then passed slowly through the Vomitoxin immunoaffinity column. The toxins bind to the antibodies in the column and the immunoaffinity column is then washed to remove other unrelated substances that have not been bound. The toxins are then eluted with eluent and injected into an analytical instrument for detection.

2. Components of the kit:

Each kit contains Zearalenone/Deoxynivalenol/Ochratoxin A 3-in-1 immunoaffinity columns of various specifications and 1 instruction manual.

3. Necessary items not provided in the box:

Equipment

LC-MS/MS
Centrifuge capable of at least 3,000-4,000RPM
Nitrogen gas evaporatorapparatus
Nitrogen gas tank and pressureregulator
Air-pressure controller bracket
Air pump
Balance with 0.01greadability
High-speed homogenizer (maximum speed> 10,000 RPM) or shaker
Grinder
Sievingscreen:2-mm
Graduated cylinder: 100 mL/10mL
Funnel: 50mL
Syringe: 10 mL/20mL
Pipette: 1 mL and pipettetips
Homogenization flask (or 250-mL conical flask withpestle)
Sample bottles andtubes
Rapid qualitative filterpaper
Microfiber filter paper (e.g. Whatman934-AH)
Column holder and syringe connector plug (for use with immunoaffinitycolumns)

3.2 Reagents

Methanol (CH₃OH): Chromatographicgrade
Acetic acid (CH₃COOH): ChromatographyGrade
Acetonitrile (CH₃CN): AnalyticalGrade
Sodium chloride (NaCl): AnalyticalGrade
TWEEN-20 (C₅₈H₁₁₄O₂₆): AnalyticalGrade
Hydrochloric acid (HCl): AnalyticalGrade
Potassium dihydrogen phosphate (KH₂PO4): AnalyticalGrade
Disodium hydrogen phosphate dodecahydrate (Na₂HPO₄12H₂O):Analytical Grade
Distilled/deionizedwater

4. Note:

Allow the immunoaffinity column to return to room temperature(22 to 25°C) before
The affinity column should be stored at 2 to 8°C, do not
Dot use any expired immunoaffinity
The sample volume can be increased or decreased appropriately as required, and the volume of the extraction solution should be adjusted
Columncapacity

Toxin name	Column Capacity: ng
ZEN	2000
DON	2000
OTA	100

When the content of the toxin in the sample divided by the dilution factor is higher than the column capacity, it is necessary to reduce the volume of the sample solution appropriately, and retest.

The pH of the loading solution of the affinity column should be within the range of 6 to 8. If it deviates from this range, the pH should be adjusted with dilute hydrochloric acid or dilute sodium hydroxide.
Maintaining consistency (such as polarity, pH, and concentration) between the test solvent loaded into any analytical instrument and the mobile phase can help eliminate any adverse solvent
WARNING: Zearalenone, Deoxynivalenol and Ochratoxin A are toxic and carcinogenic; protective equipment such as gloves and masks should always be used during
Vessels and tools used to handle toxin solutions should be completely immersed in a sodium hypochlorite solution (5% v/v) overnight.
Ensure the LC-MS/MS is clean, and the tubing is primed appropriately for each
Follow appropriate instrument precautions if using

5. Storage conditions and period of validity

Storage Conditions: 2 to 8°C

Expiry Date: this product is valid for a period of 18 months.