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    Immunoaffinity Column 3-in-1 Zearalenone/Deoxynivalenol/Ochratoxin A

    The immunoaffinity column can selectively adsorb Zearalenone (ZEN),Deoxynivalenol(DON) and Ochratoxin A (OTA) from the sample solution, thereby having a highly targeted purification effect on the sample. The sample solution that has been purified by passing through  the affinity column can be directly used for HPLC or  LC-MS/MS analysis after concentrating with nitrogen gas and resuspended.

    Order No.: HCM6225B/HCM6250B

    1. Principle:

    The basis of the measurement is the antigen-antibody reaction. Antibodies are connected to the column and the toxins in the sample are extracted, filtered, and diluted, and then passed slowly through the Vomitoxin immunoaffinity column. The toxins bind to the antibodies in the column and the immunoaffinity column is then washed to remove other unrelated substances that have not been bound. The toxins are then eluted with eluent and injected into an analytical instrument for detection.

    2. Components of the kit:

    Each kit contains  Zearalenone/Deoxynivalenol/Ochratoxin  A 3-in-1 immunoaffinity columns of various specifications and 1 instruction manual.

    3. Necessary items not provided in the box:

    • Equipment
    • LC-MS/MS
    • Centrifuge capable of at least 3,000-4,000RPM
    • Nitrogen gas evaporatorapparatus
    • Nitrogen gas tank and pressureregulator
    • Air-pressure controller bracket
    • Air pump
    • Balance with 0.01greadability
    • High-speed homogenizer (maximum speed> 10,000 RPM) or shaker
    • Grinder
    • Sievingscreen:2-mm
    • Graduated cylinder: 100 mL/10mL
    • Funnel: 50mL
    • Syringe: 10 mL/20mL
    • Pipette: 1 mL and pipettetips
    • Homogenization flask (or 250-mL conical flask withpestle)
    • Sample bottles andtubes
    • Rapid qualitative filterpaper
    • Microfiber filter paper (e.g. Whatman934-AH)
    • Column holder and syringe connector plug (for use with immunoaffinitycolumns)

    3.2 Reagents

    • Methanol (CH3OH): Chromatographicgrade
    • Acetic acid (CH3COOH): ChromatographyGrade
    • Acetonitrile (CH3CN): AnalyticalGrade
    • Sodium chloride (NaCl): AnalyticalGrade
    • TWEEN-20 (C58H114O26): AnalyticalGrade
    • Hydrochloric acid (HCl): AnalyticalGrade
    • Potassium dihydrogen phosphate (KH2PO4): AnalyticalGrade
    • Disodium hydrogen phosphate dodecahydrate (Na2HPO412H2O):Analytical Grade
    • Distilled/deionizedwater

    4. Note:

    • Allow the immunoaffinity column to return to room temperature(22 to 25°C) before
    • The affinity column should be stored at 2 to 8°C, do not
    • Dot use any expired immunoaffinity
    • The sample volume can be increased or decreased appropriately as required, and the volume of the extraction solution should be adjusted
    • Columncapacity

     

    Toxin nameColumn Capacity: ng
    ZEN2000
    DON2000
    OTA100

    When the content of the toxin in the sample divided by the dilution factor is higher than the column capacity, it is necessary to reduce the volume of the sample solution appropriately, and retest.

    • The pH of the loading solution of the affinity column should be within the range of 6 to 8. If it deviates from this range, the pH should be adjusted with dilute hydrochloric acid or dilute sodium hydroxide.
    • Maintaining consistency (such as polarity, pH, and concentration) between the test solvent loaded into any analytical instrument and the mobile phase can help eliminate any adverse solvent
    • WARNING: Zearalenone, Deoxynivalenol and Ochratoxin A are toxic and carcinogenic; protective equipment such as gloves and masks should always be used during
    • Vessels and tools used to handle toxin solutions should be completely immersed in a sodium hypochlorite solution (5% v/v) overnight.
    • Ensure the LC-MS/MS is clean, and the tubing is primed appropriately for each
    • Follow appropriate instrument precautions if using

     

    5. Storage conditions and period of validity

    Storage Conditions: 2 to 8°C

    Expiry Date: this product is valid for a period of 18 months.

     

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