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    Aflatoxins B1 ELISA Test Kit for Rice Flour, Dairy and Feed

    Intended Use

    The Aflatoxin B1 ELISA Test Kit is a competitive ELISA for the quantitative analysis of Aflatoxin B1 in dairy products, rice flour, etc.

    Brochure- Dairy Test Ver.2021-3

    Meizheng Bio-Tech Company Overview-V2022-1 2022.6.14

    Summary

    Aflatoxins are toxic and carcinogenic. They are metabolites of the fungi Aspergillus flavus and Aspergillus parasiticus. There are four principal types of aflatoxin: B1, B2, G1 and G2, which are named for their respective innate fluorescent properties. Aflatoxin B1 is the most frequently encountered of the group and the most toxic one. Aflatoxins can be found mainly in milk, cereals, nuts, cottonseed and human blood.

    Meizheng Bio-Tech Validated Matrixes

    Soybean milk powder, milk powder, rice protein powder, milk, rice flour, yogurt, edible oil, and feed.

    Use Principles

    The Aflatoxin B1 kit is an indirect competitive enzyme-labeled immunoassay. The Aflatoxin B1 antigen is precoated on the wells. The precoated antigen compete the Aflatoxin B1 antibody (antibody solution) with Aflatoxin B1 in the sample, anti-Aflatoxin B1 antibody binds to the Aflatoxin B1-HRP enzyme conjugate. Then pipette the substrate solution to the wells to convert the color.

    The color of unknown samples is compared to the color of the standards and the Aflatoxin B1 concentration of the samples is derived.

    Cross Reactivity

    Aflatoxin B1 ………………………………………………….100%

    Aflatoxin B2 ………………………………………………….38%

    Aflatoxin G1 ………………………………………………….27%

    Aflatoxin G2 ………………………………………………….4%

    Aflatoxin M1 …………………………………………………20%

    Aflatoxin M2 …………………………………………………<1%

    Materials Required But Not Provided

    1. Devices:

    ---- Microwell plate Reader (450nm/630nm)

    ---- Oscillator / Vortex

    ---- Centrifuge

    ---- Pipette: 10mL

    ---- Balance (sensibility: 0.01g)

    ---- Rubber suction bulb

    ---- Centrifuge tube: 2mL, 10mL, 50mL

    ---- Micropipette: Single channel pipette (10-100µL, 100-1000µL)

    Multi-channel pipette (30-300µL)

    1. Reagent:

    ---- Methanol (AR)

    ---- Deionized water

    ---- Concentrated sulfuric acid (18 mol/L)

    Provided Materials and Reagent

    Component96 wells
    Antigen coated plate12 × 8 wells
    6 vials of Standard1mL each
    AFB1 Enzyme Conjugate12mL
    AFB1 Antibody Solution6mL
    Substrate A6mL
    Substrate B6mL
    Concentrated Sample Diluting Buffer40mL
    Concentrated Wash Buffer (10×)40mL
    Package Insert1
    Plate Cover Sheet2

     

    Testing Report1

    Note: The concentrations of the standard are 0ng/kg, 5ng/kg, 15ng/kg, 45ng/kg, 135ng/kg, 405ng/kg.

    Quantity

    1-10, 11-100, 101-1000, 1001-10000

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