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    Immunoaffinity Column for Aflatoxin B1

    The immunoaffinity column can selectively adsorb aflatoxin B1 from the sample solution, thereby purifying the sample. The purified sample solution can then be directly used for HPLC or LC-MS/MS analysis.

    Immunoaffinity columns can be used in combination with HPLC or LC-MS/MS to achieve rapid testing, and to increase signal-to-noise ratio and improve the accuracy of the detection method.


    Aflatoxins are toxic metabolites of a class of fungi (such as Aspergillus flavus and Aspergillus parasiticus). They are highly carcinogenic and are found mainly in grains, peanuts, nuts, cottonseeds, animal feed, vegetable oils, as well as animal tissues and blood. Among them, Aflatoxin B1 (AFT B1) ranks first in terms of toxicity, carcinogenicity and frequency of contamination.


    The basis of the measurement is the antigen-antibody reaction. Antibodies are connected to the column and the aflatoxin in the sample is extracted, filtered, and diluted, and then passed slowly through the aflatoxin immunoaffinity column. The toxins bind to the antibodies in the column and the immunoaffinity column is then washed to remove other unrelated substances that have not been bound. Aflatoxin is then eluted with methanol and injected into an analytical instrument for detection.

    Order No.HCM0325B
    Sample TypeCorn, wheat, nuts, corn oil, sunflower seed oil, feed, flour, wheat flour, chili, pepper, soybean paste, sesame oil and other vegetable oils, straw, peanuts, Chinese medicine (19 types), black tea, green tea, oolong tea, and Pu’er tea.
    Packaging25 Tests
    Column Capacity300 ng
    Volume of Column3 ml
    StorageThe storage temperature is 2-8˚C.
    Shelf lifeThe validity for this kit is 18 months
    Lead timeShipped within 5-12 working days.

    Provided Materials and Reagent

    1. Aflatoxin immunoaffinity columns: 25 T
    2. 1 instruction manual.

    Materials Required But Not Provided


    • Derivatization device: such as optical derivatization device, photochemical derivatization device, iodine derivatization device
    • Nitrogen gas evaporator apparatus
    • Nitrogen gas tank and pressure regulator
    • Air-pressure controller bracket
    • Air pump
    • Balance with 0.001g readability
    • High-speed homogenizer (maximum speed> 10,000 RPM) or shaker
    • Grinder
    • Sieving screen:1-mm
    • Graduated cylinder: 100 mL/10 mL
    • Funnel: 50 mL
    • Syringe: 10 mL/20 mL
    • Pipette: 1 mL and pipette tips
    • Homogenization flask (or 250-mL conical flask with pestle)
    • Vials and tubes
    • Rapid qualitative filter paper
    • Microfiber filter paper (e.g.: Whatman 934-AH)
    • Column holder and syringe connector plug (for use with immunoaffinity columns)


    • Methanol (CH3OH): Analytical Grade
    • Methanol (CH3OH): Analytical grade forextraction/chromatography grade for elution
    • Acetonitrile (CH3CN): Analytical Grade
    • Sodium chloride (NaCl): Analytical Grade
    • TWEEN-20 (C58H114O26): Analytical Grade
    • Hydrochloric acid (HCl): Analytical Grade
    • Sodium hydroxide (NaOH): Analytical Grade
    • Distilled or deionized water

    Note: The pictures shown are sample product pictures.

    For the actual product, please check Provided Materials and Reagent or consult the sales manager.


    1-10, 11-100, 101-1000, 1001-10000


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