Background:
Aflatoxins are toxic and carcinogenic. They are metabolites of the fungi Aspergillus flavus and Aspergillus parasiticus. There are four principle types of aflatoxin: B1, B2, G1 and G2, which are named for their respective innate fluorescent properties. Aflatoxin B1 is the most frequently encountered of the group and the most toxic one. Aflatoxins can be found mainly in cereals, nuts, cottonseed and human blood.
Aflatoxin B1 is a potent human carcinogen, and may contribute to human liver cancer.
| Manufacturer | Meizheng |
| Trademark | ToxinFast® |
| Order No. | HEM0396 |
| Analyte | Aflatoxins B1 |
| Sample Type | Corn, rice, wheat, beans, peanut, peanut butter, edible oil and food or feed made of grain |
| Methodology | Indirect competitive enzyme-labeled immunoassay. |
| Packaging | 96 Wells |
| Storage | The kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE! |
| Shelf life | The validity for this kit is 12 months |
| Lead time | Shipped within 5-12 working days. |
| Cross Reactivity | AFB1 100% AFB2 38% AFG1 27% AFG2 4% AFM1 20% AFM2 <1% |
Calculate Result:
Please use the professional analysis instruments of Elisa kit for calculation, which is more accurate and fast to get result for kinds of samples
Sensitivity, accuracy and precision
Sensitivity: 10ng/kg
Limit of detection
Rice flour…………………………………… 120ng/kg
Milk………………………………………………………………………… 50ng/kg
Percent Recovery, %
Rice flour and milkl: 90±15%
Precision (CV %):
Intra-lab assay: CV%≤10%
Provided Materials and Reagent:
| Component | 96 wells |
| Antigen coated plate | 12 test strips of 8 wells each |
| 5 vials of Standard | 1mL each |
| Enzyme Conjugate | 6mL |
| Antibody Solution | 6mL |
| Substrate A | 6mL |
| Substrate B | 6mL |
| Concentrated sample diluting buffer | 40mL |
| Concentrated Wash Buffer (10×) | 40mL |
| Package Insert | 1 |
| Plate Cover Sheet | 2 |
| Testing Report | 1 |
Note: The concentrations of the standards are 0μg/kg, 0.03μg/kg, 0.12μg/kg, 0.48μg/kg and 2μg/kg respectively.
Materials Required But Not Provided
- Devices:
—- Microwell plate Reader (450nm/630nm)
—- Oscillator
—- Vortex
—- Centrifuge /funnel
—- Balance (sensibility: 0.01g)
—- Micropipette: Single channel pipette (10µL-100µL, 100µL-1000µL)
—-Multi-channel pipette (30µL-300µL)
—- Pipette/ triangular flask/ beaker
—- Rubber suction bulb
—- Centrifuge tube (7mL, 50mL)
—- Quantitative analysis filter paper
2. Reagent:
—- Methanol (AR)
—- Deionized water
—- Acetonitrile (AR)
—- Concentrated sulfuric acid (18mol/L)
Note: The pictures shown are sample product pictures.
For the actual product, please check Provided Materials and Reagent or consult the sales manager.
