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Food and Feed Mycotoxin Aflatoxins B1 ELISA Test Kit

The Aflatoxin B1 ELISA Test Kit is a competitive ELISA for the qualitative or quantitative analysis of Aflatoxin B1 in corn, rice, wheat, beans, peanut, peanut butter, edible oil and food or feed made of grain.

Brochure-Dairy Test. PDF

Meizheng Bio-Tech Company Overview. PDF


Aflatoxins are toxic and carcinogenic. They are metabolites of the fungi Aspergillus flavus and Aspergillus parasiticus. There are four principle types of aflatoxin: B1, B2, G1 and G2, which are named for their respective innate fluorescent properties. Aflatoxin B1 is the most frequently encountered of the group and the most toxic one. Aflatoxins can be found mainly in cereals, nuts, cottonseed and human blood.

Aflatoxin B1 is a potent human carcinogen, and may contribute to human liver cancer.

Order No.HEM0396
AnalyteAflatoxins B1
Sample TypeCorn, rice, wheat, beans, peanut, peanut butter, edible oil and food or feed made of grain
MethodologyIndirect competitive enzyme-labeled immunoassay.
Packaging96 Wells
StorageThe kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE!
Shelf lifeThe validity for this kit is 12 months
Lead timeShipped within 5-12 working days.
Cross ReactivityAFB1 100%
AFB2 38%
AFG1 27%
AFG2 4%
AFM1 20%
AFM2 <1%

 Calculate Result:

Please use the professional analysis instruments of Elisa kit for calculation, which is more accurate and fast to get result for kinds of samples

Sensitivity, accuracy and precision

Sensitivity:           10ng/kg

Limit of detection

Rice flour…………………………………… 120ng/kg

Milk………………………………………………………………………… 50ng/kg

Percent Recovery, %

Rice flour and milkl: 90±15%

Precision (CV %):

Intra-lab assay: CV%≤10%

Provided Materials and Reagent:

Component96 wells
Antigen coated plate12 test strips of 8 wells each
5 vials of Standard1mL each
Enzyme Conjugate6mL
Antibody Solution6mL
Substrate A6mL
Substrate B6mL
Concentrated sample diluting buffer40mL
Concentrated Wash Buffer (10×)40mL
Package Insert1
Plate Cover Sheet2
Testing Report1

 Note: The concentrations of the standards are 0μg/kg, 0.03μg/kg, 0.12μg/kg, 0.48μg/kg and 2μg/kg respectively.

Materials Required But Not Provided

  1. Devices:

—- Microwell plate Reader (450nm/630nm)

—- Oscillator

—- Vortex

—- Centrifuge /funnel

—- Balance (sensibility: 0.01g)

—- Micropipette: Single channel pipette (10µL-100µL, 100µL-1000µL)

—-Multi-channel pipette (30µL-300µL)

—- Pipette/ triangular flask/ beaker

—- Rubber suction bulb

—- Centrifuge tube (7mL, 50mL)

—- Quantitative analysis filter paper

2. Reagent:

—- Methanol (AR)

—- Deionized water

—- Acetonitrile (AR)

—- Concentrated sulfuric acid (18mol/L)

Note: The pictures shown are sample product pictures.

For the actual product, please check Provided Materials and Reagent or consult the sales manager.


1-10, 11-100, 101-1000, 1001-10000


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The immunoaffinity column can selectively adsorb aflatoxins (B1, B2, G1, G2) from the sample solution, thereby purifying the sample. The purified sample solution can then be directly used for HPLC or LC-MS/MS analysis.Affinity columns can be used in combination with HPLC or LC-MS/MS to achieve rapid testing, and to increase signal-noise ratio  and improve the accuracy of the detection method.

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