- Purpose:
The immunoaffinity column can selectively adsorb fumonisin (FB1, FB2, FB3) from the sample solution, thereby having a highly targeted purification effect on the sample. The sample solution that has been purified by passing through the column can be directly used for LC- MS/MS analysis after concentrating with nitrogen gas and resuspended or for HPLC analysis after derivatization.
Affinity columns can be used in combination with HPLC or LC- MS/MS to achieve rapid testing, and to increase signal-to-noise ratio and improve the accuracy of the detection method.
2. Overview:
Fumonisin is a metabolite produced by Fusarium fungi. It has acute and chronic toxicities and is species-specific and organ specific. Studies have found that high concentrations of fumonisin can cause acute species- specific toxicity symptoms in many domestic and laboratory animals, such as equine leukoencephalomalacia, porcine pulmonary edema, and liver and kidney diseases in sheep. The toxin has also been found to be related to esophageal and liver cancer in humans.
3. Principle:
The basis of the measurement is the antigen-antibody reaction. Antibodies are connected to the column and the fumonisin in the sample is extracted, filtered, and diluted, and then passed slowly through the Ochratoxin A immunoaffinity column. The toxins bind to the antibodies in the column and the immunoaffinity column is then washed to remove other unrelated substances that have not been bound. Fumonisin is then eluted with methanol and injected into an analytical instrument for detection.
4. Components of the kit:
Each kit contains fumonisin immunoaffinity columns of various specifications and 1 instruction manual.
5. Necessary items not provided in the box:
- Equipment
- HPLC
- Derivatization device: such as optical derivatization device, photochemical derivatization device, iodine derivatizationdevice
- Centrifuge capable of at least 3,000-4,000 xg
- Nitrogen gas evaporatorapparatus
- Nitrogen gas tank and pressureregulator
- Air-pressure controllerbracket
- Airpump
- Balance with 0.01greadability
- High-speed homogenizer (maximum speed> 10,000 r/ min) or shaker
- Grinder
- Sievingscreen:2-mm
- Graduated cylinder: 100 mL/10mL
- Funnel: 50mL
- Syringe: 10 mL/20mL
- Pipette: 1 mL and pipettetips
- Homogenization flask (or 250-mL conical flask withpestle)
- Sample bottles andtubes
- Rapid qualitative filterpaper
- Microfiber filter paper (e.g., Whatman934-AH)
- Column holder and syringe connector plug (for use with immunoaffinity columns)
- 5.1 Reagents
- Methanol (CH3OH): Analytical grade forextraction/Chromatography grade for elution
- Disodium hydrogen phosphate dodecahydrate (Na2HPO4∙ 12H2O):Analytical Grade
- Acetic acid (CH3COOH): Analyticalgrade
- Acetonitrile (CH3CN): Analytical grade forextraction/Chromatography grade for elution
- Potassium dihydrogen phosphate (KH2PO4): AnalyticalGrade
- Sodium chloride (NaCl): AnalyticalGrade
- TWEEN-20 (C58H114O26): AnalyticalGrade
- Hydrochloric acid (HCl): AnalyticalGrade
- Sodium hydroxide (NaOH): AnalyticalGrade
- Potassium chloride (KCl): AnalyticalGrade
- Distilled or deionizedwater
6. Note:
- Allowthe immunoaffinity column to return to room temperature (22 to 25°C) before
- The affinity column should be stored at 2 to 8°C, do not
- Dot use any expired immunoaffinity
- The sample volume can be increased or decreased appropriately as required, and the volume of the extraction solution should be adjusted
- Columncapacity
Toxin name Column Capacity: ng FB1 5000
FB2 FB3
