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Immunoaffinity Column for Mycotoxins 6-in-1 Combo

The immunoaffinity column can simultaneously adsorb Aflatoxin (AFT B1, AFT B2, AFT G1, AFT G2, AFT M1, AFT M2), Zearalenone

(ZEN) and its derivatives (ZAN, α-ZOL, β-ZOL, α-ZAL, β-ZAL), Deoxynivalenol (DON) and 15-Acetyldeoxynivalenol (15-ADON), Ochratoxin (OTA), T-2 toxin and HT-2 toxin, fumonisin (FB1, FB2, FB3) from the sample extract solution, thereby having a highly targeted purification effect on the above 20 toxins of the six major types, and the samples that have be purified after passing through the column can be used in LC-MS (LC-MS/MS) analysis.

Order No.: HCM3110

  1. Overview:

The basis of the measurement is the antigen-antibody reaction. 20 antibodies of six main toxins are connected to the column concurrently. After the sample is extracted and filtered, it is slowly passed through the immunoaffinity column. The toxins bind to the corresponding antibodies in the column. The immunoaffinity column is then washed to remove other unrelated substances that have not been bound. Elute the toxin with eluent and then inject into the analytical instrument for detection.

Order No. : HCM3110;

Product Properties: AFT B1/B2/G1/G2/M1/M2: 300, FB1/FB2/FB3: 5000, OTA: 100, DON/15-ADON: 2000, ZEN: 1000, T-2/HT-2:1000;

Specifications: 6ml,10T;

Samples: Grain,feed,etc.

  1. Components of the kit:

Each kit contains 6 in 1 immunoaffinity columns and 1 instruction manual.

  1. Necessary items not provided in the box:

3.1 Equipment


Nitrogen gas evaporator apparatus

Nitrogen gas tank and pressure regulator

Air-pressure controller bracket

Air pump

Balance with 0.01g readability

High-speed homogenizer (maximum speed> 10,000 r/ min) or shaker


Sieving screen:2-mm

Graduated cylinder: 100 mL/10 mL

Funnel: 50 mL

Syringe: 10 mL/20 mL

Pipette: 1 mL and pipette tips

Homogenization flask (or 250-mL conical flask with pestle)

Vials and tubes

Rapid qualitative filter paper

Microfiber filter paper (e.g., Whatman 934-AH)

Column holder and syringe connector plug (for use with immunoaffinity columns)

3.2 Reagents

  • Methanol (CH3OH): Chromatography Grade
  • Acetonitrile (CH3CN): Chromatography Grade
  • Sodium chloride (NaCl): Analytical Grade
  • TWEEN-20 (C58H114O26): Analytical Grade
  • Acetic acid (CH3COOH): Analytical Grade
  • Sodium hydroxide (NaOH): Analytical Grade
  • Potassium chloride (KCl): Analytical Grade
  • Potassium dihydrogen phosphate (KH2PO4): Analytical Grade
  • Disodium hydrogen phosphate dodecahydrate (Na2HPO4·12H2O): Analytical Grade
  • Distilled or deionized water
  1. Note:
  • Allow the immunoaffinity column to return to room temperature (22 to 25°C) before use.
  • The affinity column should be stored at 2 to 8°C, do not freeze.
  • Dot use any expired immunoaffinity column.
  • The sample volume can be increased or decreased appropriately as required, and the volume of the extraction solution should be adjusted accordingly.
  • The pH of the loading solution of the affinity column should be within the range of 6 to 8. If it deviates from this range, the pH should be adjusted with dilute hydrochloric acid or dilute sodium hydroxide.
  • Maintaining consistency (such as polarity, pH, and concentration) between the test solvent loaded into any analytical instrument and the mobile phase can help eliminate any adverse solvent effects.
  • Column capacity:
Toxin nameColumn capacity (ng) Toxin name Column capacity (ng)
AFT B1300ZEN1000


  • Aflatoxin, Zearalenone, Vomitoxin , Ochratoxin,T-2 toxins and Fumonisin, as well as the derivatives of these toxins are toxic; and gloves should be worn when handling them.
  • The used container and toxin solution is best soaked with sodium hypochlorite solution (5%V/V) overnight
  • Ensure the LC-MS/MS is clean and the tubing is primed appropriately for each run.

1-10, 11-100, 101-1000, 1001-10000


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