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    Tetracyclines ELISA Test Kit for Animal Tissue, Seafood and Egg

    Qualitative or quantitative analysis of tetracycline, oxytetracycline, aureomycin, doxycycline in animal tissue, seafood and egg, etc.

    Meizheng Bio-Tech Company Overview. PDF

    Background:

    Tetracyclines are antibiotics which inhibit the bacterial growth by stopping protein synthesis. They have been widely used for the past forty years as therapeutic agent in human and veterinary medicine but also as growth promoter in animal husbandry. But the abuse of antibiotics will lead to the residues in the livestock and indirectly affect the health of human being.

    HPLC method is now widely used in the detection of tetracyclines residues, but its high cost and complex operation make it unacceptable.

    Use Principles: 

    Tetracyclines (TCs) ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The tetracycline antigen is precoated on the wells. The precoated antigen compete the tetracycline antibody (antibody solution) with tetracycline in the sample, anti- tetracycline antibody binds to the tetracycline-HRP enzyme conjugate. Then pipet the substrate solutions to the wells to convert the color.

    The color of unknown samples is compared to the color of the standards and the tetracycline concentration of the samples is derived.

    ManufacturerMeizheng
    Order No.ME09A
    AnalyteTetracyclines
    Sample TypeAnimal tissue, seafood and egg, etc.
    MethodologyIndirect competitive enzyme-labeled immunoassay.
    Packaging96 Wells
    StorageThe kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE!
    Shelf lifeThe validity for this kit is 12 months
    Lead timeShipped within 5-12 working days.
    Cross ReactivityTetracycline…………………………………100%
    Doxycycline…………………………………90%
    Chlortetracycline……………………..……180%
    Oxytetracycline……………………………70%
     Calculate ResultAnalyze the result by using the software
    Sensitivity 0.2ppb
    Limit of detectionTissue, egg……………………………………5ppb
    Percent Recovery, %Tissue, egg……………………………90±20%
    Precision (CV %):Intra-lab assay: CV%≤10%

    Provided Materials and Reagent

    Component96 wells
    Antigen coated plate12 test strips of 8 wells each
    6 vials of standard2mL each
    TCs Enzyme Conjugate6mL
    TCs Antibody Solution6mL
    Substrate A6mL
    Substrate B6mL
    Concentrated Wash Buffer (10×)40mL
    Concentrated sample extracting buffer40mL
    Concentrated sample diluting buffer40mL
    IFU1
    Testing Report1
    Plate Cover Sheet2
    Antigen coated plate12 test strips of 8 wells each

    Note:The concentrations of the standards are 0ppb, 0.2ppb, 0.6ppb, 1.8ppb, 5.4ppb and 16.2ppb.

    Materials Required But Not Provided

    1. Devices:

    ---- Microwell plate Reader (450nm/630nm)

    ---- Homogenizer

    ---- Oscillator

    ---- Vortex

    ---- Centrifuge

    ---- Balance (sensibility: 0.01g)

    ---- Transfer pipette: 10mL

    ---- Aurilave

    ---- Centrifuge tube: 5mL/7mL, 50mL

    ---- Micropipette: Single channel pipette (10µL-100µL, 100µL-1000µL)

    Multi-channel pipette (300µL)

    1. Reagent:

    ---- Deionized water

    ---- Methanol (AR)

    ---- Concentrated sulfuric acid (18mol/L)

     Note: The pictures shown are sample product pictures.

    For the actual product, please check Provided Materials and Reagent or consult the sales manager.

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