The basis of the measurement is the antigen-antibody reaction. More than 20 antibodies of seven main toxins are connected to the column concurrently. After the sample is extracted and filtered, it is slowly passed through the immunoaffinity column. The toxins bind to the corresponding antibodies in the column. The immunoaffinity column is then washed to remove other unrelated substances that have not been bound. Elute the toxin with eluent and then inject into the analytical instrument for detection.
Order No. : HCM4210;
Product Properties: AFT B1/B2/G1/G2/M1/M2: 300, FB1/FB2/FB3: 5000, OTA: 100, ST:300, DON/15-ADON: 2000, ZEN: 1000, T-2/HT-2:1000;
- Components of the kit:
Each kit contains 7 in 1 immunoaffinity columns and 1 instruction manual.
Necessary items not provided in the box:
Nitrogen gas evaporator apparatus
Nitrogen gas tank and pressure regulator
Air-pressure controller bracket
Balance with 0.01g readability
High-speed homogenizer (maximum speed> 10,000 r/ min) or shaker
Graduated cylinder: 100 mL/10 mL
Funnel: 50 mL
Syringe: 10 mL/20 mL
Pipette: 1 mL and pipette tips
Homogenization flask (or 250-mL conical flask with pestle)
Sample bottles and tubes
Rapid qualitative filter paper
Microfiber filter paper (e.g. Whatman 934-AH)
Column holder and syringe connector plug (for use with immunoaffinity columns)
Methanol (CH3OH): Chromatography Grade
Acetic acid (CH3COOH): Analytical Grade
Acetonitrile (CH3CN): Analytical Grade
Sodium chloride (NaCl): Analytical Grade
TWEEN-20 (C58H114O26): Analytical Grade
Sodium hydroxide (NaOH): Analytical Grade
Potassium chloride (KCl): Analytical Grade
Potassium dihydrogen phosphate (KH2PO4): Analytical Grade
Disodium hydrogen phosphate dodecahydrate (Na2HPO4·12H2O): Analytical Grade
Distilled or deionized water
Allow the immunoaffinity column to return to room temperature(22 to 25°C) before use.
The affinity column should be stored at 2 to 8°C, do not freeze.
Dot use any expired immunoaffinity column.
The sample volume can be increased or decreased appropriately as required, and the volume of the extraction solution should be adjusted accordingly.
The pH of the loading solution of the affinity column should be within the range of 6 to 8. If it deviates from this range, the pH should be adjusted with dilute hydrochloric acid or dilute sodium hydroxide.
Maintaining consistency (such as polarity, pH, and concentration) between the test solvent loaded into any analytical instrument and the mobile phase can help eliminate any adverse solvent effects.
- Column capacity:
|Toxin name||Column capacity (ng)||Toxin name||Column capacity (ng)|
Aflatoxin, Zearalenone, Vomitoxin, Ochratoxin, T-2 toxins, sterigmatocystin and Fumonisin, as well as the derivatives of these toxins are toxic; and gloves should be worn when handling them.
The used container and toxin solution is best soaked with sodium hypochlorite solution (5%V/V) overnight.
Ensure the LC-MS/MS is clean and the tubing is primed appropriately for each run.
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