This MaxSignal Aflatoxin M1 Immunoaffinity Column can selectively extract Aflatoxin M1 from the sample solution, thereby purifying the sample. The purified sample solution can be directly used for HPLC or LC-MSMS analysis after being blown dried using nitrogen gas and re-dissolved in suitable solvent.
Aflatoxins are toxic secondary-metabolites of a class of fungi (such as Aspergillus flavus and Aspergillus parasiticus). They are highly carcinogenic and are found mainly in grains, peanuts, nuts, cottonseeds, animal feed, vegetable oils, as well as animal tissues and blood. Among them, Aflatoxin B1 ranks first in terms of toxicity, carcinogenicity and frequency of contamination. Aflatoxin M1 is the hydroxylated metabolite of Aflatoxin B1 and a strong carcinogen. Cow’s milk and its products are prone to Aflatoxin M1 contamination.
The basis of the IAC measurement is the antigen-antibody interaction. The antibody is embedded in the column. After a sample is extracted and filtered, it is slowly passed through the IAC. The toxoids bind to their corresponding antibody in the column. The IAC is then washed to remove unbound substances. The toxins are then eluted with the eluent, concentrated using nitrogen gas, then injected into an analytical instrument for detection. Affinity columns can be used in combination with HPLC and LC-MSMS to achieve rapid testing, and to increase signal-to-noise ratio and improve the accuracy of the detection method.
This kit is manufactured to the international quality standard ISO 9001.
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Combination of Immunoaffinity Column with high performance liquid chromatography (HPLC) or LC-MS/MS is a quantitative method for the detection of mycotoxins in samples such as grains, nuts, feed, dairy and more. Samples are purified by the column and then analyzed with HPLC or LC-MSMS.