This MaxSignal Zearalenone Immunoaffinity Column can selectively extract Zearalenone from the sample solution, thereby having a highly targeted purification effect on the sample. The sample solution that has been purified by passing through the column can be directly used for HPLC or LC-MS/MS analysis after being concentrated with nitrogen gas and resuspended.
Zearalenone (ZEN), also known as F-2 toxin, is mainly produced by Fusarium strains. Its components are 2,4-dihydroxybenzoic acid lactones produced by Fusarium graminearum and Fusarium culmorum. ZEN mainly pollutes crops such as corn, wheat, and grains. It has a strong estrogenic effect and can cause hyperestrogenism, as well as severe reproductive tract symptoms and infertility, and is also immunotoxic and genotoxic.
The basis of the IAC measurement is the antigen-antibody reaction. Antibodies are connected to the column and the toxins in the sample are extracted, filtered, and passed slowly through the zearalenone immunoaffinity column. The toxins bind to the antibodies in the column and the immunoaffinity column is then washed to remove other unrelated substances that have not been bound. Zearalenone is eluted with methanol, and then injected into the analytical instrument for detection.
This kit is manufactured to the international quality standard ISO 9001.
|Product Brand Name||MaxSignal|
|Quantity in a Package Amount||25.0 Box|
|Sample Type||Grain, Feed|
|Sample Volume||3 mL|
|Shipping Condition||Blue Ice|
Combination of Immunoaffinity Column with high performance liquid chromatography (HPLC) or LC-MS/MS is a quantitative method for the detection of mycotoxins in samples such as grains, nuts, feed, dairy and more. Samples are purified by the column and then analyzed with HPLC or LC-MSMS.