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Milk and Honey Antibiotic Beta-lactams ELISA Test Kit

Qualitative or quantitative analysis of Beta-lactams concentrations in milk and honey.

Order No.: SC0166


Beta-lactams ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Penicillin and other beta-lactams such as ampicillin, amoxicillin, cloxacillin, oxacillin in milk sample. Penicillin is among the most widely used antibiotics for the treatment of bacterial infections in man and animals. Penicillin is also administered to animals in feed for growth promotion and for collective prophylactic treatment. The monitoring of penicillin residues in edible tissues and milk is important because of the hypersensitivity of some individuals to these antibiotics and also the emergence of antibiotic-resistant strains of bacteria. For this reason, maximum residue limits have been specified for food products and milk to try and control the levels of these antibiotics reaching the consumer.

Use Principles

Beta-lactams ELISA Test Kit is an indirect competitive enzyme-labeled immunoassay. The Penicillin G antigen is precoated on the wells. The precoated antigen compete the Penicillin G antibody (antibody solution) with Penicillin G in the sample, anti-Penicillin G antibody binds to the Penicillin G-HRP enzyme conjugate. Then pipette the substrate solutions to the wells to convert the color.

The color of unknown samples is compared to the color of the standards and the Penicillin G concentration of the samples is derived.

【Cross Reactivity】

Penicillin G 100%

Ampicillin > 100%

Cefoperazone > 100%

Ceftiofur > 100%

Cloxacillin > 100%

Nafcillin > 100%

Oxacillin > 100%

Amoxicillin 44%

【Materials Required But Not Provided】

  1. Devices:

—- Microwell Plate Reader (450nm/630nm)

—- Oscillator / Vortex

—- Centrifuge

—- Balance (sensibility: 0.01g)

—- Centrifuge tube: 2mL, 7mL

—- Micropipette: Single channel pipette (10-100µL, 100-1000µL)

Multi-channel pipette (30-300µL)

  1. Reagent:

—- Deionized water

—- 18M concentrated sulfuric acid (H2SO4)

【Provided Materials and Reagent】

Component96 wellsStorage
Antigen coated plate12 × 8 wells2-8℃
Penicillin G Powder Stock (1000ng) *2 vials-20℃
Empty vials for standards
Negative control (white cap tube)1 vial2-8℃
0.08μg/kg (yellow cap tube)1 vial2-8℃
0.2μg/kg (orange cap tube)1 vial2-8℃
0.4μg/kg (pink cap tube)1 vial2-8℃
0.8μg/kg (purple cap tube)1 vial2-8℃
1.2μg/kg (blue cap tube)1 vial2-8℃
Concentrated Wash Buffer (20×)28mL2-8℃
Beta-lactams Capture Protein (10×)1.8mL-20℃
Beta-lactams Capture Protein Diluent12mL-20℃
Antibody #2 Diluent20mL2-8℃
HRP-Conjugated Antibody #2 (200×)100μL-20℃
TMB Substrate12mL2-8℃
Milk Standard Dilution Buffer 115mL-20℃
Beta-lactams Extraction Buffer (10×)2 × 25mL2-8℃
Package Insert1
Testing Report1
Plate Cover Sheet2


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