Search

100% Secure delivery without contacting the courier

    In Stock

    Milk and Honey Antibiotic Beta-lactams ELISA Test Kit

    Qualitative or quantitative analysis of Beta-lactams concentrations in milk and honey.

    Order No.: SC0166

    【Summary】

    Beta-lactams ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Penicillin and other beta-lactams such as ampicillin, amoxicillin, cloxacillin, oxacillin in milk sample. Penicillin is among the most widely used antibiotics for the treatment of bacterial infections in man and animals. Penicillin is also administered to animals in feed for growth promotion and for collective prophylactic treatment. The monitoring of penicillin residues in edible tissues and milk is important because of the hypersensitivity of some individuals to these antibiotics and also the emergence of antibiotic-resistant strains of bacteria. For this reason, maximum residue limits have been specified for food products and milk to try and control the levels of these antibiotics reaching the consumer.

    Use Principles

    Beta-lactams ELISA Test Kit is an indirect competitive enzyme-labeled immunoassay. The Penicillin G antigen is precoated on the wells. The precoated antigen compete the Penicillin G antibody (antibody solution) with Penicillin G in the sample, anti-Penicillin G antibody binds to the Penicillin G-HRP enzyme conjugate. Then pipette the substrate solutions to the wells to convert the color.

    The color of unknown samples is compared to the color of the standards and the Penicillin G concentration of the samples is derived.

    【Cross Reactivity】

    Penicillin G 100%

    Ampicillin > 100%

    Cefoperazone > 100%

    Ceftiofur > 100%

    Cloxacillin > 100%

    Nafcillin > 100%

    Oxacillin > 100%

    Amoxicillin 44%

    【Materials Required But Not Provided】

    1. Devices:

    ---- Microwell Plate Reader (450nm/630nm)

    ---- Oscillator / Vortex

    ---- Centrifuge

    ---- Balance (sensibility: 0.01g)

    ---- Centrifuge tube: 2mL, 7mL

    ---- Micropipette: Single channel pipette (10-100µL, 100-1000µL)

    Multi-channel pipette (30-300µL)

    1. Reagent:

    ---- Deionized water

    ---- 18M concentrated sulfuric acid (H2SO4)

    【Provided Materials and Reagent】

    Component96 wellsStorage
    Antigen coated plate12 × 8 wells2-8℃
    Penicillin G Powder Stock (1000ng) *2 vials-20℃
    Empty vials for standards
    Negative control (white cap tube)1 vial2-8℃
    0.08μg/kg (yellow cap tube)1 vial2-8℃
    0.2μg/kg (orange cap tube)1 vial2-8℃
    0.4μg/kg (pink cap tube)1 vial2-8℃
    0.8μg/kg (purple cap tube)1 vial2-8℃
    1.2μg/kg (blue cap tube)1 vial2-8℃
    Concentrated Wash Buffer (20×)28mL2-8℃
    Beta-lactams Capture Protein (10×)1.8mL-20℃
    Beta-lactams Capture Protein Diluent12mL-20℃
    Antibody #2 Diluent20mL2-8℃
    HRP-Conjugated Antibody #2 (200×)100μL-20℃
    TMB Substrate12mL2-8℃
    Milk Standard Dilution Buffer 115mL-20℃
    Beta-lactams Extraction Buffer (10×)2 × 25mL2-8℃
    Package Insert1-
    Testing Report1-
    Plate Cover Sheet2-

    Reviews

    There are no reviews yet.

    Write a review

    Your email address will not be published.

    Bestsellers

    Neomycin/Paromomycin, Kanamycin, Gentamicin and Spectinomycin Milk Comb Rapid Test Kit

    (0 Reviews)
    The Neomycin/Paromomycin, Kanamycin, Gentamicin and Spectinomycin Rapid Test Kit is a lateral flow assay that determines a qualitative level for the presence of neomycin/ paromomycin, kanamycin, gentamicin and spectinomycin antibiotic residues in raw cow’s milk.Brochure-Dairy Test. PDFMeizheng Bio-Tech Company Overview. PDF

    Milk Albendazole Metabolite Rapid Test Kit

    (0 Reviews)
    The Albendazole Metabolite Rapid Test Kit is a lateral flow assay that determines a qualitative level for the presence of albendazole metabolite antibiotic residues in raw cow’s milk.Brochure-Dairy Test. PDFMeizheng Bio-Tech Company Overview. PDF

    Aflatoxins B1 ELISA Test Kit for Feed

    (0 Reviews)
    The Aflatoxin B1 ELISA Test Kit is a competitive ELISA for the qualitative or quantitative analysis of aflatoxin B1 in feed and feed material.Brochure-Grains & Feed Test. PDFMeizheng Bio-Tech Company Overview. PDF

    Milk Carbaryl Lateral Flow Rapid Test Kit

    (0 Reviews)
    The Carbaryl Rapid Test Kit is a lateral flow assay that determines a qualitative level for the presence of carbaryl in raw cow milk. This test is designed for rapid field use or daily control in laboratories.Brochure-Dairy Test. PDFMeizheng Bio-Tech Company Overview. PDF

    Trimethoprim ELISA Test Kit for Animal Tissues, Aquatic Tissue and Eggs

    (0 Reviews)
    Qualitative or quantitative analysis of trimethoprim (TMP) residues in animal tissues, aquatic tissue and eggs.Meizheng Bio-Tech Company Overview. PDF

    Grains and Feed Mycotoxin Aflatoxin B1 Qualitative Rapid Test Kit

    (0 Reviews)
    The kit is a lateral flow immunochromatographic assay that determines a qualitative level for the presence of aflatoxins B1 and is intended for use in grains, feed materials and feed products. This method is suitable for the rapid on-site test.

    Antibiotic Tylosin Honey ELISA Test Kits

    $180.00$257.14
    (0 Reviews)
    Tylosin is a macrolide antibiotic mainly used to prevent livestock and poultry pathogens and mycoplasma infections.

     

    Back to Top
    Product has been added to your cart