Background:
Streptomycin is an antibiotic (antimycobacterial) drug, the first of a class of drugs called aminoglycosides to be discovered, and it was the first effective treatment for tuberculosis. It is derived from the actinobacterium streptomyces griseus. Streptomycin is a bactericidal antibiotic.
Adverse effects of this medicine are ototoxicity, nephrotoxicity, fetal auditory toxicity, and neuromuscular paralysis.
Use Principles：
Streptomycin (STR) ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The streptomycin antigen is precoated on the wells. The precoated antigen compete the streptomycin antibody (antibody solution) with streptomycin in the sample, anti-streptomycin antibody binds to the streptomycin-HRP enzyme conjugate. Then pipet the substrate solutions to the wells to convert the color.
The color of unknown samples is compared to the color of the standards and the streptomycin concentration of the samples is derived.
| Manufacturer | Meizheng |
| Order No. | SC0045 |
| Analyte | Streptomycin |
| Sample Type | Honey, milk, etc. |
| Methodology | Indirect competitive enzyme-labeled immunoassay. |
| Packaging | 96 Wells |
| Storage | The kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE! |
| Shelf life | The validity for this kit is 12 months |
| Lead time | Shipped within 5-12 working days. |
| Cross Reactivity | Streptomycin 100% Dihydrostreptomycin Sulphate 100% Kanamycin ＜0.01% Gentamycin ＜0.01% |
| Calculate Result | Analyze the result by using the software |
| Sensitivity | 0.2ppb |
| Limit of detection | Raw milk and pure milk 4ppb Honey……. 2ppb |
| Percent Recovery, % | Raw milk and pure milk 100±20% Honey 100±20% |
| Precision (CV %): | Intra-lab assay: CV<10% |
Provided Materials and Reagent
| Component | 96T |
| Antigen coated plate | 96 wells |
| 6 vials of standard | 1mL each |
| High concentration standard (100ppb) | 1mL |
| Enzyme Conjugate | 6mL |
| Antibody Solution | 6mL |
| Substrate A | 6mL |
| Substrate B | 6mL |
| Concentrated wash buffer (10×) | 40mL |
| Concentrated sample diluting buffer (2×) | 40mL |
| Concentrated derivatization reagent (10×) | 1mL each |
| IFU | 1 |
| Testing Report | 1 |
| Plate cover sheet | 2 |
Note: The concentrations of the standards are 0ppb, 0.2ppb, 0.6ppb, 1.8ppb and 5.4ppb.
Materials Required But Not Provided
- Devices:
—- Microwell plate Reader (450nm/630nm)
—- Balance (sensibility: 0.01g)
—- Centrifuge
—- Vortex
—- Centrifuge tube: 2mL, 10mL, 50mL
—- Graduated pipette: 10mL
—-Micropipette: Single channel pipette (10µL-100µL, 100µL-1000µL)
Multi-channel pipette (30-300µL)
- Reagent:
—- Deionized water
—- Concentrated sulfuric acid (18mol/L)
Note: The pictures shown are sample product pictures.
For the actual product, please check Provided Materials and Reagent or consult the sales manager.
