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    Milk and Honey Veterinary Drugs Doxycycline ELISA Test Kit

    Qualitative or quantitative analysis of Doxycycline residues in milk and honey.

    Order No.: SC0051

    【Summary】

    Doxycycline (DOXY) is a high-efficiency broad-spectrum antibiotic with strong inhibitory effect on a variety of pathogenic bacteria, and is often used in the treatment of various infectious diseases in animals. However, its residues in animal meat, eggs and other foods are a serious threat to human health, and can cause diseases such as aplastic anemia and agranulocytosis. Low concentrations of drug residues can also induce drug resistance of pathogenic bacteria.

    【Principles】

    Doxycycline ELISA Test Kit is an indirect competitive enzyme-labeled immunoassay. The Doxycycline antigen is precoated on the wells. The precoated antigen compete the Doxycycline antibody (antibody solution) with sulfonamides in the sample, anti-Doxycycline antibody binds to the Doxycycline-HRP enzyme conjugate. Then pipet the substrate solutions to the wells to convert the color.

    The color of unknown samples is compared to the color of the standards and the Doxycycline concentration of the samples is derived.

    Cross-reactivity

    Doxycycline…………………………………….………….100%

    【Materials Required But Not Provided】

    1. Devices:

    ---- Microwell Plate Reader (450nm/630nm)

    ---- Oscillator / Vortex

    ---- Constant Temperature Incubator

    ---- Centrifuge

    ---- Balance (sensibility: 0.01g)

    ---- Graduated pipette: 10mL

    ---- Rubber suction bulb

    ---- Centrifuge tube: 10mL, 50mL

    ---- Micropipette: Single channel pipette (10-100μL, 100-1000μL)

    Multi-channel pipette (30-300μL)

    1. Reagent:

    ---- Deionized water

    ---- 18M concentrated sulfuric acid (H2SO4)

    【Provided Materials and Reagent】

    Component 96 wells
    Antigen coated plate12 × 8 wells
    6 vials of standard2mL each
    Enzyme Conjugate6mL
    Antibody Solution6mL
    Substrate A6mL
    Substrate B6mL
    Concentrated Wash Buffer (10×)40mL
    Concentrated Sample Diluting Buffer40mL
    Package Insert1
    Testing Report1
    Plate Cover Sheet2

    Note: The concentrations of the standards are 0μg/kg, 0.1μg/kg, 0.3μg/kg, 0.9μg/kg, 2.7μg/kg and 8.1μg/kg.

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