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    Milk Qualitative or Quantitative Gentamicin ELISA Test Kit

    Qualitative or quantitative analysis of gentamicin residues in raw milk.

    Order No.: SC0046

    【Summary】

    Gentamicin (GEN) belongs to aminoglycoside antibiotics, mainly acting on Gram-negative bacteria. It is widely used in veterinary clinics and animal feed additives due to its broad antibacterial spectrum, good efficacy, and low price. However, the serum effective concentration of GEN is very similar to the toxic concentration, and the safety range is small. It is easy to produce toxic and side effects during its application. Especially, the use of large doses is not standardized, which may easily lead to excessive GEN residues in animal food and pose a hazard to human health. Severe damage to cranial nerves, hearing and kidneys.

    【Principles】

    Gentamicin ELISA Test Kit is an indirect competitive enzyme-labeled immunoassay. The gentamicin antigen is precoated on the wells. The precoated antigen compete the gentamicin antibody (antibody solution) with sulfonamides in the sample, anti-gentamicin antibody binds to the gentamicin-HRP enzyme conjugate. Then pipette the substrate solutions to the wells to convert the color.

    The color of unknown samples is compared to the color of the standards and the gentamicin concentration of the samples is derived.

    Cross Reactivity

    Gentamicin 100%

    【Materials Required But Not Provided】

    1. Devices:

    ---- Microwell Plate Reader (450nm/630nm)

    ---- Oscillator / Vortex

    ---- Constant Temperature Incubator

    ---- Centrifuge

    ---- Balance (sensibility: 0.01g)

    ---- Graduated pipette: 10mL

    ---- Rubber suction bulb

    ---- Centrifuge tube: 10mL, 50mL

    ---- Micropipette: Single channel pipette (10-100μL, 100-1000μL)

    Multi-channel pipette (30-300μL)

    1. Reagent:

    ---- Deionized water

    ---- 18M concentrated sulfuric acid (H2SO4)

    【Provided Materials and Reagent】

    Component 96 wells
    Antigen coated plate12 × 8 wells
    5 vials of standard1mL each
    High Concentration Standard (1ppm)1mL
    Enzyme Conjugate6mL
    Antibody Solution9mL
    Substrate A6mL
    Substrate B6mL
    Concentrated Wash Buffer (10×)40mL
    Concentrated Sample Diluting Buffer40mL
    Package Insert1
    Testing Report1
    Plate Cover Sheet2

    Note: The concentrations of the standards are 0μg/kg, 0.2μg/kg, 0.6μg/kg, 1.8μg/kg, and 5.4μg/kg.

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