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    Milk Qualitative or Quantitative Sulfonamides ELISA Test Kit

    Qualitative or quantitative analysis of sulfonamides in raw milk and finished milk.

    Order No.: SC0047

    【Summary】

    Sulfonamides (SAs) are group of synthetic antibiotic agents that have been widely used for therapeutic and prophylactic purposes in both animals and human. They are used as feed additives as well, mainly for fattening of calves and pigs. SAs have seen an emergence in the aquaculture industry due to their effectiveness against gram-negative and gram-positive organisms.

    The Sulfonamides (SAs) ELISA Kit produced by Meizheng significantly reduces both the testing cost and the operating errors.

    【Use Principles】

    Sulfonamides ELISA Test Kit is an indirect competitive enzyme-labeled immunoassay. The sulfonamides antigen is precoated on the wells. The precoated antigen compete the sulfonamides antibody (antibody solution) with sulfonamides in the sample, anti- sulfonamides antibody binds to the sulfonamides-HRP enzyme conjugate. Then pipette the substrate solutions to the wells to convert the color.

    The color of unknown samples is compared to the color of the standards and the sulfonamides concentration of the samples is derived.

    【Cross Reactivity】

    Sulfamethazine  100%

    Sulfadiazine  797%

    Sulfamonomethoxine  1360%

    Sulfaquinoxaline 120%

    Sulfachloropyridazine 170%

    Sulfachloropyrazine sodium 2113%

    Sulfadimoxine  1054%

    Sulfamerazine  335%

    Sulfadoxine  1057%

    Sulfadimethoxine  849%

    Phthalylsulfathiazole  90%

    Sulfamoppyridine 62%

    Sulfapyridine 55%

    Sulfamethizole 518%

    Sulfamidinum 49%

    Sulfamethoxazole 21%

    【Materials Required But Not Provided】

    1. Devices:

    ---- Microwell Plate Reader (450nm/630nm)

    ---- Oscillator / Vortex

    ---- Constant Temperature Incubator

    ---- Balance (sensibility: 0.01g)

    ---- Centrifuge tube: 2mL, 10mL

    ---- Micropipette: Single channel pipette (10-100µL, 100-1000µL)

    Multi-channel pipette (30 -300µL)

    1. Reagent:

    ---- Deionized water

    ---- 18M concentrated sulfuric acid (H2SO4)

    【Provided Materials and Reagent】

    Component 96 wells
    Antigen coated plate12 × 8 wells
    5 vials of standard1ml each
    High Concentration Standard (1ppm)1ml
    Enzyme Conjugate6ml
    Antibody Solution6ml
    Substrate A6ml
    Substrate B6ml
    Concentrated Wash Buffer (10×)40ml
    Concentrated Sample Diluting Buffer40ml
    Package Insert1
    Testing Report1
    Plate cover sheet2

    Note: The concentrations of the standards are 0ug/kg, 2ug/kg, 6ug/kg, 18ug/kg and 54ug/kg.

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