Thiamphenicol (TAP), another name for thiamphenicol, is an amide alcohol antibiotic. Thiamphenicol is a congener of chloramphenicol. Its antibacterial spectrum and antibacterial effect are similar to chloramphenicol. It has broad-spectrum antimicrobial effect and is illegally added in animal husbandry. Although thiamphenicol inhibits red blood cells, white blood cells and platelets to a lesser extent than chloramphenicol, its immunosuppressive effect is stronger than that of chloramphenicol.
Thiamphenicol ELISA Test Kit is an indirect competitive enzyme-labeled immunoassay. The thiamphenicol antigen is precoated on the wells. The precoated antigen compete the thiamphenicol antibody (antibody solution) with thiamphenicol in the sample, anti-thiamphenicol antibody binds to the thiamphenicol-HRP enzyme conjugate. Then pipette the substrate solutions to the wells to convert the color.
The color of unknown samples is compared to the color of the standards and the thiamphenicol concentration of the samples is derived.
【Materials Required But Not Provided】
---- Microwell Plate Reader (450nm/630nm)
---- Constant Temperature Incubator
---- Micropipette: Single channel pipette (10-100μL, 100-1000μL)
Multi-channel pipette (30-300μL)
---- Deionized water
---- 18M concentrated sulfuric acid (H2SO4)
【Provided Materials and Reagent】
|Antigen coated plate||12 × 8 wells|
|6 vials of standard||0.5mL each|
|High Concentration Standard (1ppm)||1mL|
|Concentrated Wash Buffer (10×)||40mL|
|Plate Cover Sheet||2|
Note: The concentrations of the standards are 0μg/kg, 2μg/kg, 6μg/kg, 18μg/kg, 54μg/kg, and 162μg/kg.