Oxytetracycline is a high-efficiency broad-spectrum antibiotic with strong inhibitory effect on a variety of pathogenic bacteria and is often used in the treatment of various infectious diseases in animals.
The Oxytetracycline ELISA Test Kit produced by Meizheng is fast, easy-to-use, accurate and has high sensitivity, with 45 minutes operation time, which can minimize the operating error and working intensity.
Oxytetracycline ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The Oxytetracycline antigen is precoated on the wells. The precoated antigen compete the Oxytetracycline antibody (Antibody Solution) with Oxytetracycline in the sample, Oxytetracycline antibody binds to the Oxytetracycline Enzyme Conjugate. After the color development of TMB Substrate, the absorbance value of the sample is negatively correlated with the Oxytetracycline contained in it. Compared with the standard curve and multiplied by its corresponding dilution factor, the content of Oxytetracycline in the sample can be calculated.
【Scope of Application】
Qualitative or quantitative analysis of Oxytetracycline in animal tissue (chicken, pork, fish), egg.
【Materials Required But Not Provided】
—- Microplate Reader (450nm/630nm filter)
—- Centrifuge tube: 5mL/7mL, 50mL
—- Graduated Pipette: 10mL
—- Rubber Bulb Syringe
—- Balance (sensibility: 0.01g)
—- Micropipette: Single channel pipette (10-100µL, 100-1000µL) Multi-channel pipette (30-300µL)
—- Deionized or distilled water
—- Methanol (AR)
—- Concentrated Hydrochloric Acid (HCl) (AR)
—- 18M Concentrated Sulfuric Acid (H2SO4)
【Provided Materials and Reagent】
|Antigen Coated Plate||96T|
|6 vials of Standard||2mL each|
|Concentrated Sample Diluting Buffer||40mL|
|Concentrated Sample Extracting Buffer||40mL|
|Concentrated Wash Buffer (10×)||40mL|
|Plate Cover Sheet||2|
Note: The concentrations of the standards are 0ppb, 0.3ppb, 0.9ppb, 2.7ppb, 8.1ppb, 24.3ppb.
Note: The pictures shown are sample product pictures.
For the actual product, please check Provided Materials and Reagent or consult the sales manager.