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Poultry and Seafood Antibiotic Lincomycin ELISA Test Kit

Qualitative or quantitative analysis of Lincomycin in tissue of poultry and seafood.

Summary

Lincomycin is a Lincosamides antibiotic mainly used to restrain synthesis of bacterial protein. Lincomycin residual in livestock and poultry will hurt liver and cause allergic reaction and Cardiovascular reaction.

Order No.: ME35AER

LOD: 0.2 ppb

Package: 96T

Sample: Meat, Seafood

Use Principles

Lincomycin ELISA Test Kit is an indirect competitive enzyme-labeled immunoassay. The Lincomycin antigen is precoated on the wells. The precoated antigen compete the Lincomycin antibody (antibody solution) with Lincomycin in the sample, anti- Lincomycin antibody binds to the Lincomycin -HRP enzyme conjugate. Then pipete the substrate solutions to the wells to convert the color.

The color of unknown samples is compared to the color of the standards and the Lincomycin concentration of the samples is derived.

Cross-reactivity

Lincomycin ………………………………………………100%

Clindamycin ………………………………………………<1%

Spiramycin…………………………………………………<1%

Erythromycin………………………………………………<1%

Tylosin…………………………………………………………<1%

Streptomycin………………………………………………<1%

Kanamycin……………………………………………………<1%

Spectinomycin………………………………………………<1%

Materials Required But Not Provided

  1. Devices:

—- Microwell plate Reader (450nm/630nm)

—- Homogenizer

—- Oscillator

—- Vortex

—- Centrifuge

—- Balance (sensibility: 0.01g)

—- Transfer pipette: 10mL

—- Aurilave

—- Centrifuge tube: 5mL, 7mL, 50mL

—-Micropipette: Single channel pipet (10µL-100µL, 100µL-1000µL)

Multi-channel pipet (30µL -300µL)

  1. Reagent:

—- Deionized water

—- Concentrated sulfuric acid (18 mol/L)

Provided Materials and Reagent

Component96 wells
Antigen coated plate12 test strips of 8 wells each
6 vials of standard1mL each
High concentration standard (2ppm)1mL each
Enzyme Conjugate6mL
Antibody Solution6mL
Substrate A6mL
Substrate B6mL
Concentrated Wash Buffer (10×)40mL
Concentrated sample diluting buffer (2×)50mL
Package Insert1
Testing Report1
Plate Cover Sheet2

Note: The concentrations of the standards are 0ppb, 0.2ppb, 0.6ppb, 1.8ppb, 5.4ppb and 16.2ppb.

Note: The pictures shown are sample product pictures.

For the actual product, please check Provided Materials and Reagent or consult the sales manager.

Quantity

1-10, 11-100, 101-1000, 1001-10000

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