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Streptomycin (TCs) ELISA Test Kit for Animal Tissue, Seafood and Egg

Qualitative or quantitative analysis of Streptomycin (TCs) in animal/aquatic tissue, etc.

Meizheng Bio-Tech Company Overview. PDF

Background:

Streptomycin is an antibiotic (antimycobacterial) drug, the first of a class of drugs called aminoglycosides to be discovered, and it was the first effective treatment for tuberculosis. It is derived from the actinobacterium Streptomyces griseus. Streptomycin is a bactericidal antibiotic. Adverse effects of this medicine are ototoxicity, nephrotoxicity, fetal auditory toxicity, and neuromuscular paralysis.

Use Principles: 

Streptomycin (TCs) ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The Streptomycin antigen is precoated on the wells. The precoated antigen compete the Streptomycin antibody (antibody solution) with Streptomycin in the sample, anti- Streptomycin antibody binds to the Streptomycin-HRP enzyme conjugate. Then pipette the substrate solutions to the wells to convert the color.

The color of unknown samples is compared to the color of the standards and the Streptomycin concentration of the samples is derived.

ManufacturerMeizheng
Order No.ME10A
AnalyteStreptomycin (TCs)
Sample TypeAnimal tissue, seafood and egg, etc.
MethodologyIndirect competitive enzyme-labeled immunoassay.
Packaging96 Wells
StorageThe kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE!
Shelf lifeThe validity for this kit is 12 months
Lead timeShipped within 5-12 working days.
Cross ReactivityStreptomycin…………………………………100%
Dihydrostreptomycin Sulphate……………100%
Kanamycin………………………………<0.01%
Gentamycin………………………………<0.01%
 Calculate ResultAnalyze the result by using the software
Sensitivity 0.2ppb
Limit of detectionTissues…………………………………………10ppb
Percent Recovery, %Tissues……………………………………90±15%
Precision (CV %):Intra-lab assay: CV%≤10%

Provided Materials and Reagent

Component96 wells
Antigen coated plate12 test strips of 8 wells each
6 vials of standard1.5mL each
High concentration standard (1ppm)1.5mL
TCs Enzyme Conjugate6mL
TCs Antibody Solution6mL
Substrate A6mL
Substrate B6mL
Concentrated Wash Buffer (10×)40mL
Package Insert1
Testing Report1
Plate Cover Sheet2

Note:The concentrations of the standards are 0ppb, 0.2ppb, 0.6ppb, 1.8ppb, 5.4ppb and 16.2ppb.

Materials Required But Not Provided

  1. Devices:

—- Microwell plate Reader (450nm/630nm)

—- Homogenizer

—- Oscillator

—- Vortex

—- Centrifuge

—- Balance (sensibility: 0.01g)

—- Transfer pipette: 10mL

—- Aurilave

—- Centrifuge tube: 5mL/7mL, 50mL

—- Micropipette: Single channel pipet (10µL-100µL, 100µL-1000µL)

Multi-channel pipet (30µL-300µL)

  1. Reagent:

—- Deionized water

—- Sodium carbonate anhydrous (AR)

—- Sodium bicarbonate (AR)

—- Concentrated sulfuric acid (18 mol/L)

 Note: The pictures shown are sample product pictures.

For the actual product, please check Provided Materials and Reagent or consult the sales manager.

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