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    Sulfonamides (SAs) ELISA Kit for Animal Tissue and Eggs

    Qualitative or quantitative analysis of sulfonamides residues in animal tissue, eggs, swine urine, etc.

    Meizheng Bio-Tech Company Overview. PDF

    Background:

    Sulfonamides (SAs) are group of synthetic antibiotic agents that have been widely used for therapeutic and prophylactic purposes in both animals and human. They are used as feed additives as well, mainly for fattening of calves and pigs. SAs have seen an emergence in the aquaculture industry due to their effectiveness against gram-negative and gram-positive organisms.

    HPLC/GC-MS method is now widely used in the detection of sulfonamides residues, but its high cost and complex operation make it unacceptable.

    Use Principles: 

    Sulfonamides (SAs) ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The sulfonamides antigen is precoated on the wells. The precoated antigen compete the sulfonamides antibody (antibody solution) with sulfonamides in the sample, anti- sulfonamides antibody binds to the sulfonamides-HRP enzyme conjugate. Then pipet the substrate solutions to the wells to convert the color.

    The color of unknown samples is compared to the color of the standards and the sulfonamides concentration of the samples is derived.

    ManufacturerMeizheng
    Order No.ME08C
    AnalyteSulfonamides
    Sample TypeAnimal tissue, eggs, swine urine, etc.
    MethodologyIndirect competitive enzyme-labeled immunoassay.
    Packaging96 Wells
    StorageThe kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE!
    Shelf lifeThe validity for this kit is 12 months
    Lead timeShipped within 5-12 working days.
    Cross ReactivitySulfamerazine……………………………100%
    Sulfadiazine………………………………155%
    Sulfamonomethoxine……………………408%
    Sulfaquinoxaline.....................................23%
    Sulfachloropyridazine............................51%
    Sulfaclozine..............................................40%
    Sulfadimethoxine....................................242%
    Sulfameter................................................253%
    Sulfamethazine........................................38%
    Sulfadoxine…………………………………285%
    Phthalylsulfathiazole (PST)………………42%
    Sulfamethoxypyridazine……………....…40%
    Sulfisomidine.........................................98%
    Sulfamethizole.........................................125%
    Sulfathiazole.............................................41%
    Sulfabenzamide.......................................39%
     Calculate ResultAnalyze the result by using the software
    Sensitivity 0.5ppb
    Limit of detectionMethod 1(Tissue samples)………………1ppb

    Method 1(eggs)…………………..………2ppb

    Method 2……………………………..….……5ppb

    Percent Recovery, %Method 1…….……………………………90±20%

    Method 2…………………………………100±30%

    Precision (CV %):Intra-lab assay: CV%≤10%

    Provided Materials and Reagent

    Component96 wells
    Antigen coated plate12 test strips of 8 wells each
    5 vials of standard1mL each
    High concentration standard 1ppm1mL each
    SAs Enzyme Conjugate6mL
    SAs Antibody Solution6mL
    Substrate A6mL
    Substrate B6mL
    Concentrated Wash Buffer (10×)40mL
    Concentrated sample diluting buffer I40mL
    Concentrated sample diluting buffer II40mL
    Concentrated sample extracting buffer15 mL
    Sample extractant1 bag
    IFU1

    Note:The concentrations of the standards are 0ppb, 0.5ppb, 1.5ppb, 4.5ppb and 13.5ppb.

    Materials Required But Not Provided

    1. Devices:

    ---- Microwell plate Reader (450nm/630nm)

    ---- Oscillator

    ---- Vortex

    ---- Rotary evaporator/nitrogen evaporator

    ---- Centrifuge

    ---- Centrifuge tube: 2mL, 10mL/ 7mL, 50mL

    ---- Balance (sensibility: 0.01g)

    ---- Graduated pipette: 10mL

    ---- Micropipette: Single channel pipet (10µL-100µL, 100µL-1000µL)

    Multi-channel pipet (30µL-300µL)

    1. Reagent:

    ---- Methanol (AR)

    ---- N-hexane (AR)

    ---- Deionized water

    -----Ethyl acetate (AR)

    -----Neutral alumina (100-200mesh)

    -----Acetonitrile (AR)

    ---- Concentrated sulfuric acid (18 mol/L)

     Note: The pictures shown are sample product pictures.

    For the actual product, please check Provided Materials and Reagent or consult the sales manager.

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