Trimethoprim (TMP) is a common sulfonamide synergist because of its weak antibacterial activity. In combination with sulfonamides, the antibacterial activity can be enhanced several times, even dozons of times. TMP can treat infection caused by escherichia coli induced septicemia, typhoid fever, cholera, secondary respiratory infection.
Trimethoprim (TMP) ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The Trimethoprim (TMP) antigen is precoated on the wells. The precoated antigen compete the Trimethoprim (TMP) antibody (antibody solution) with Trimethoprim (TMP)s in the sample, anti- Trimethoprim (TMP) antibody binds to the Trimethoprim-HRP enzyme conjugate. Then pipet the substrate solutions to the wells to convert the color.
The color of unknown samples is compared to the color of the standards and the Trimethoprim (TMP) concentration of the samples is derived.
|Sample Type||Animal tissues, aquatic tissue and eggs.|
|Methodology||Indirect competitive enzyme-labeled immunoassay.|
|Storage||The kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE!|
|Shelf life||The validity for this kit is 12 months|
|Lead time||Shipped within 5-12 working days.|
|Cross Reactivity||Trimethoprim (TMP)………………………100%|
|Calculate Result||Analyze the result by using the software|
|Limit of detection||2ppb|
|Percent Recovery, %||100±30%|
|Precision (CV %):||Intra-lab assay: CV%＜10%|
Provided Materials and Reagent
|Antigen coated plate||12 test strips of 8 wells each|
|6 vials of standard||1.5mL each|
|High concentration standard (1ppm)||1mL|
|Concentrated Wash Buffer (10×)||40mL|
|Concentrated Auxiliary Extracting Agent (10×)||15mL|
|Concentrated Sample Diluting Buffer||40mL|
|Plate cover sheet||2|
Note:The concentrations of the standards are 0ppb, 0.5ppb, 1.5ppb, 4.5ppb, 13.5ppb and 40.5ppb.
Materials Required But Not Provided
—- Microwell plate Reader (450nm/630nm)
—- Rotary evaporator/nitrogen evaporator
—- Balance (sensibility: 0.01g)
—- Centrifuge tube: 5mL/7mL, 50mL
—- Transfer pipette: 10mL
—- Micropipette: Single channel pipet (10µL-100µL, 100µL-1000µL)
Multi-channel pipet (30µL -300µL)
—- Deionized water
—- Ethyl acetate (AR)
—- N-hexane (AR)
—- Concentrated sulfuric acid (18 mol/L)
Note: The pictures shown are sample product pictures.
For the actual product, please check Provided Materials and Reagent or consult the sales manager.