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Verterinary Drugs Residues Furacilin Metabolite ELISA Test Kit

Qualitative or quantitative analysis of Furacilin Metabolite in samples of poultry tissues (including fish, shrimp, chicken, pork, liver, etc), eggs etc.

Summary

Nitrofurans, which are effective against a wide range of bacteria, include furazolidone, nitrofurazone, nitrofurantoin and related compounds. The food and drug administration has prohibited all uses of nitrofuran drugs in food-producing animals because they pose a public health risk. Evidence indicates that the drugs may induce carcinogenic residues in animal tissues.

Order No.: 0.03 ppb

Package: 96T

Samples: Meat, Seafood, Liver, Egg

Use Principles

Furacilin Metabolite ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The Furacilin Metabolite antigen is precoated on the wells. The precoated antigen compete the Furacilin Metabolite antibody (Antibody Solution) with Furacilin Metabolite in the sample, Furacilin Metabolite antibody binds to the Furacilin Metabolite Enzyme Conjugate. After the color development of TMB Substrate, the absorbance value of the sample is negatively correlated with the Furacilin Metabolite contained in it. Compared with the standard curve and multiplied by its corresponding dilution factor, the content of Furacilin Metabolite in the sample can be calculated.

Cross-reactivity

Furacilin Metabolite…………………………………………100%

Furaltadone Metabolite……………………………………<0.1%

Furantoin Metabolite…………………………………………<0.1%

Furazolidone Metabolite……………………………………<0.1%

Materials Required But Not Provided

  1. Devices:

—- Microplate Reader (450nm/630nm filter)

—- Vortex

—- Centrifuge

—- Homogenizer

—- Constant Temperature Incubator

—- Balance (sensibility: 0.01g)

—- Termovap Sample Concentrator

—- Water Bath

—- Graduated Pipette: 10mL

—- Rubber Bulb Syringe

—- Centrifuge tube: 10mL, 50mL

—- Micropipette: Single channel pipette (10-100µL, 100-1000µL)

Multi-channel pipette (30-300µL)

  1. Reagent:

—- Ethyl acetate (AR)

—- Deionized water

—- N-hexane (AR)

—- Methanol (AR)

—- Concentrated hydrochloric acid (HCl) (AR)

—- -Sodium hydroxide (NaOH) (AR)

—- Dipotassium hydrogen phosphate trihydrate (K2HPO4‧3H2O) (AR)

—- 18M concentrated sulfuric acid (H2SO4)

[Provided Materials and Reagent]

Component96 wells
Antigen Coated Plate96T
6 vials of Standard1mL each
High Concentration Standard (100ppb)1mL
Enzyme Conjugate6mL
Antibody Solution6mL
Substrate A6mL
Substrate B6mL
Concentrated Wash Buffer (10×)40mL
Concentrated Sample Diluting Buffer (2×)40mL
Concentrated Derivatization Reagent (10×)1mL
Package Insert1
Testing Report1
Plate Cover Sheet2

Note: The concentrations of the standards are 0ppb, 0.03ppb, 0.09ppb, 0.27ppb, 0.81ppb and 2.43ppb.

Note: The pictures shown are sample product pictures.

For the actual product, please check Provided Materials and Reagent or consult the sales manager.

Quantity

1-10, 11-100, 101-1000, 1001-10000

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