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    Aflatoxins B1 ELISA Test Kit for Feed

    The Aflatoxin B1 ELISA Test Kit is a competitive ELISA for the qualitative or quantitative analysis of aflatoxin B1 in feed and feed material.

    Brochure-Grains & Feed Test. PDF

    Meizheng Bio-Tech Company Overview. PDF

    Background:

    Aflatoxins are toxic and carcinogenic. They are metabolites of the fungi Aspergillus flavus and Aspergillus parasiticus. There are four principle types of aflatoxin: B1, B2, G1 and G2, which are named for their respective innate fluorescent properties. Aflatoxin B1 is the most frequently encountered of the group and the most toxic one. Aflatoxins can be found mainly in cereals, nuts, cottonseed and human blood.

    Aflatoxin B1 is a potent human carcinogen, and may contribute to human liver cancer.

    ManufacturerMeizheng
    TrademarkToxinFast®
    Order No.HEM0496
    AnalyteAflatoxins B1
    Sample TypeFeed and feed material
    MethodologyIndirect competitive enzyme-labeled immunoassay.
    Packaging96 Wells
    StorageThe kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE!
    Shelf lifeThe validity for this kit is 12 months
    Lead timeShipped within 5-12 working days.
    Cross ReactivityAFB1……………………………………100%
    AFB2……………………………………38%
    AFG1……………………………………27%
    AFG2……………………………………4%
    AFM1………………..…………………20%
    AFM2……………………………………<1%
    Calculate ResultPlease use the professional analysis instruments of Elisa kit for calculation, which is more accurate and fast to get result for kinds of samples
    Limit of detection1.5µg/kg
    Limit of quantitation1.5µg/kg
    Percent Recovery, %100±20%
    Precision (CV %):Intra-lab assay: CV%≤10%

    Provided Materials and Reagent:

    ComponentQTY
    Antigen coated plate12×8 wells each
    5 vials of Standard1mL each
    AFB1 Enzyme Conjugate12mL
    AFB1 Antibody Solution12mL
    Substrate10mL*2
    Concentrated Wash buffer (10×)40mL
    Package Insert1
    Plate Cover Sheet2
    Testing Report1

    Note: The concentrations of the standards are 0µg/kg, 1.5µg/kg, 5µg/kg, 15µg/kg and 50µg/kg respectively (the real concentration of the standards is 1/25 of the set concentrations).

    Materials Required But Not Provided

    1. Devices:

    ---- Microwell plate Reader (450nm/630nm)

    ---- Oscillator/ Vortex

    ---- Thermostat incubator

    ---- Centrifuge (or quantitative filter)

    ---- Balance (sensibility: 0.01g)

    ---- Pipette: 50mL

    ---- Triangular flask (with stopper): 100mL

    ---- Centrifuge tube: 7mL

    ---- Micropipet: Single channel pipet (10µL-100µL, 100µL-1000µL)  Multi-channel pipet (30µL-300µL)

    1. Reagent:

    ---- Methanol (AR)

    ---- Deionized water

    ---- Concentrated sulfuric acid (18 mol/L)

    Note: The pictures shown are sample product pictures.

    For the actual product, please check Provided Materials and Reagent or consult the sales manager.

    Quantity

    1-10, 11-100, 101-1000, 1001-10000

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