Background:
Aflatoxins are toxic and carcinogenic. They are metabolites of the fungi Aspergillus flavus and Aspergillus parasiticus. There are four principle types of aflatoxin: B1, B2, G1 and G2, which are named for their respective innate fluorescent properties. Aflatoxin B1 is the most frequently encountered of the group and the most toxic one. Aflatoxins can be found mainly in cereals, nuts, cottonseed and human blood.
Aflatoxin B1 is a potent human carcinogen, and may contribute to human liver cancer.
| Manufacturer | Meizheng |
| Trademark | ToxinFast® |
| Order No. | HEM0496 |
| Analyte | Aflatoxins B1 |
| Sample Type | Feed and feed material |
| Methodology | Indirect competitive enzyme-labeled immunoassay. |
| Packaging | 96 Wells |
| Storage | The kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE! |
| Shelf life | The validity for this kit is 12 months |
| Lead time | Shipped within 5-12 working days. |
| Cross Reactivity | AFB1……………………………………100% AFB2……………………………………38% AFG1……………………………………27% AFG2……………………………………4% AFM1………………..…………………20% AFM2……………………………………<1% |
| Calculate Result | Please use the professional analysis instruments of Elisa kit for calculation, which is more accurate and fast to get result for kinds of samples |
| Limit of detection | 1.5µg/kg |
| Limit of quantitation | 1.5µg/kg |
| Percent Recovery, % | 100±20% |
| Precision (CV %): | Intra-lab assay: CV%≤10% |
Provided Materials and Reagent:
| Component | QTY |
| Antigen coated plate | 12×8 wells each |
| 5 vials of Standard | 1mL each |
| AFB1 Enzyme Conjugate | 12mL |
| AFB1 Antibody Solution | 12mL |
| Substrate | 10mL*2 |
| Concentrated Wash buffer (10×) | 40mL |
| Package Insert | 1 |
| Plate Cover Sheet | 2 |
| Testing Report | 1 |
Note: The concentrations of the standards are 0µg/kg, 1.5µg/kg, 5µg/kg, 15µg/kg and 50µg/kg respectively (the real concentration of the standards is 1/25 of the set concentrations).
Materials Required But Not Provided
- Devices:
—- Microwell plate Reader (450nm/630nm)
—- Oscillator/ Vortex
—- Thermostat incubator
—- Centrifuge (or quantitative filter)
—- Balance (sensibility: 0.01g)
—- Pipette: 50mL
—- Triangular flask (with stopper): 100mL
—- Centrifuge tube: 7mL
—- Micropipet: Single channel pipet (10µL-100µL, 100µL-1000µL) Multi-channel pipet (30µL-300µL)
- Reagent:
—- Methanol (AR)
—- Deionized water
—- Concentrated sulfuric acid (18 mol/L)
Note: The pictures shown are sample product pictures.
For the actual product, please check Provided Materials and Reagent or consult the sales manager.
