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Aflatoxins B1 ELISA Test Kit for Feed

The Aflatoxin B1 ELISA Test Kit is a competitive ELISA for the qualitative or quantitative analysis of aflatoxin B1 in feed and feed material.

Brochure-Grains & Feed Test. PDF

Meizheng Bio-Tech Company Overview. PDF

Background:

Aflatoxins are toxic and carcinogenic. They are metabolites of the fungi Aspergillus flavus and Aspergillus parasiticus. There are four principle types of aflatoxin: B1, B2, G1 and G2, which are named for their respective innate fluorescent properties. Aflatoxin B1 is the most frequently encountered of the group and the most toxic one. Aflatoxins can be found mainly in cereals, nuts, cottonseed and human blood.

Aflatoxin B1 is a potent human carcinogen, and may contribute to human liver cancer.

ManufacturerMeizheng
TrademarkToxinFast®
Order No.HEM0496
AnalyteAflatoxins B1
Sample TypeFeed and feed material
MethodologyIndirect competitive enzyme-labeled immunoassay.
Packaging96 Wells
StorageThe kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE!
Shelf lifeThe validity for this kit is 12 months
Lead timeShipped within 5-12 working days.
Cross ReactivityAFB1……………………………………100%
AFB2……………………………………38%
AFG1……………………………………27%
AFG2……………………………………4%
AFM1………………..…………………20%
AFM2……………………………………<1%
Calculate ResultPlease use the professional analysis instruments of Elisa kit for calculation, which is more accurate and fast to get result for kinds of samples
Limit of detection1.5µg/kg
Limit of quantitation1.5µg/kg
Percent Recovery, %100±20%
Precision (CV %):Intra-lab assay: CV%≤10%

Provided Materials and Reagent:

ComponentQTY
Antigen coated plate12×8 wells each
5 vials of Standard1mL each
AFB1 Enzyme Conjugate12mL
AFB1 Antibody Solution12mL
Substrate10mL*2
Concentrated Wash buffer (10×)40mL
Package Insert1
Plate Cover Sheet2
Testing Report1

Note: The concentrations of the standards are 0µg/kg, 1.5µg/kg, 5µg/kg, 15µg/kg and 50µg/kg respectively (the real concentration of the standards is 1/25 of the set concentrations).

Materials Required But Not Provided

  1. Devices:

—- Microwell plate Reader (450nm/630nm)

—- Oscillator/ Vortex

—- Thermostat incubator

—- Centrifuge (or quantitative filter)

—- Balance (sensibility: 0.01g)

—- Pipette: 50mL

—- Triangular flask (with stopper): 100mL

—- Centrifuge tube: 7mL

—- Micropipet: Single channel pipet (10µL-100µL, 100µL-1000µL)  Multi-channel pipet (30µL-300µL)

  1. Reagent:

—- Methanol (AR)

—- Deionized water

—- Concentrated sulfuric acid (18 mol/L)

Note: The pictures shown are sample product pictures.

For the actual product, please check Provided Materials and Reagent or consult the sales manager.

Quantity

1-10, 11-100, 101-1000, 1001-10000

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