Amantadine is a drug with multiple uses and varied mechanisms of action. Chinese poultry farmers used amantadine to protect birds against avian influenza, that cause avian flu (H5N1) strains in China and southeast Asia are now resistant to amantadine. However, in Western countries and according to international livestock regulations, amantadine is approved only for use in humans.
The Amantadine ELISA Test kit is an indirect competitive enzyme-labeled immunoassay. The antigen is precoated on the wells. The precoated antigen compete the antibody (antibody solution) with Amantadine in the sample, anti-Amantadine antibody binds to the HRP enzyme conjugate. Then pipette the substrate solution to the wells to convert the color.
The color of unknown samples is compared to the color of the standards and the Amantadine concentration of the samples is derived.
【Scope of Application】
The Amantadine ELISA Test Kit is competitive ELISA for the qualitative or quantitative analysis of Amantadine in samples of chicken and pork.
【Materials Required But Not Provided】
---- Microplate Reader (450nm/630nm filter)
---- Rubber Bulb Syringe
---- Termovap Sample Concentrator
---- Constant temperature incubator (Optional)
---- Centrifuge tube (2mL, 10mL/7mL, 50mL)
---- Balance (sensibility: 0.01g)
---- Graduated pipette: 10mL
---- Micropipette: Single channel pipette (10-100µL, 100-1000µL)
Multi-channel pipette (30-300µL)
---- Deionized water
---- Sodium Chloride (NaCl) (AR)
---- Acetonitrile (AR)
---- 18M concentrated sulfuric acid (H2SO4)
【Provided Materials and Reagent】
|Antigen coated plate||96 T|
|6 vials of Standard||1mL each|
|High concentration standard (1ppm)||1mL|
|Concentrated Wash Buffer (10×)||40mL|
|Concentrated sample diluting buffer||40mL|
|Plate Cover Sheet||2|
Note: The concentrations of the standards are 0ppb, 0.5ppb, 1.5ppb, 4.5ppb, 13.5ppb, 40.5ppb, respectively.
Note: The pictures shown are sample product pictures.
For the actual product, please check Provided Materials and Reagent or consult the sales manager.