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    Animal and Aquatic Tissue Sulfonamides ELISA Test Kit

    Qualitative or quantitative analysis of sulfonamides residues in tissue, aquatic tissue, etc.

    Summary

    Sulfonamides are group of synthetic antibiotic agents that have been widely used for therapeutic and prophylactic purposes in both animals and human. They are used as feed additives as well, mainly for fattening of calves and pigs. SAs have seen an emergence in the aquaculture industry due to their effectiveness against gram-negative and gram-positive organisms.

    HPLC/GC-MS method is now widely used in the detection of sulfonamides residues, but its high cost and complex operation make it unacceptable.

    Use Principles

    Sulfonamides ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The sulfonamides antigen is precoated on the wells. The precoated antigen compete the sulfonamides antibody (antibody solution) with sulfonamides in the sample, anti- sulfonamides antibody binds to the sulfonamides-HRP enzyme conjugate. Then pipet the substrate solutions to the wells to convert the color.

    The color of unknown samples is compared to the color of the standards and the sulfonamides concentration of the samples is derived.

    Cross-reactivity

    Sulfadiazine 100%

    Sulfadoxine 181%

    Sulfamonomethoxine 150%

    Phthalylsulfathiazole (PST) 90%

    Sulfamethoxydiazine 85%

    Sulfadimethoxypyrimidine 67%

    Benzene Sulfapyridine 62%

    Sulfapyridine 55%

    Sulfamerazine 52%

    Sulfamethizole 49%

    Sulfachloropyridazine 29.6%

    Sulfamethazine 13%

    Materials Required But Not Provided

    1. Devices:

    ---- Microwell plate Reader (450nm/630nm)

    ---- Oscillator

    ---- Vortex

    ---- Rotary evaporator/nitrogen evaporator

    ---- Centrifuge

    ---- Balance (sensibility: 0.01g)

    ---- Graduated pipette: 10mL

    ---- Micropipette: Single channel pipet (10µL-100µL, 100µL-1000µL)

    Multi-channel pipet (30µL-300µL)

    1. Reagent:

    ---- Methanol (AR)

    ---- N-hexane (AR)

    ---- Deionized water

    ---- Concentrated sulfuric acid (18 mol/L)

    Provided Materials and Reagent

    Component96 wells
    Antigen coated plate96 T
    5 vials of standard1mL each
    High concentration standard 1ppm1mL
    SAs Enzyme Conjugate6mL
    SAs Antibody Solution9mL
    Substrate A6mL
    Substrate B6mL
    Concentrated Wash Buffer (10×)40mL
    SAs Concentrated Sample Diluting Buffer I (10×)45mL
    SAs Concentrated Sample Diluting Buffer II (2×)45mL
    IFU1

    Note: The concentrations of the standards are 0ppb, 0.5ppb, 1.5ppb, 4.5ppb and 13.5ppb.

    Note: The pictures shown are sample product pictures.

    For the actual product, please check Provided Materials and Reagent or consult the sales manager.

    Quantity

    1-10, 11-100, 101-1000, 1001-10000

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