Animal Tissue and Feed Illegal Additive Ractopamine ELISA Test Kit

【Summary】

Ractopamine is a phenylethanolamine β2-adrenergic receptor agonist with a wide range of physiological effects. It is mainly used in the clinical treatment of bronchial asthma, congestive heart failure and muscular dystrophy. When the dosage is 5 to 10 times higher than the clinical dosage, it can regulate metabolism in the body, increase fat decomposition, promote protein synthesis, and show a nutrient redistribution effect. Adding it to the feed greatly improves the lean meat rate of livestock. When humans eat animal tissues containing ractopamine, it is very easy to produce toxic reactions such as rapid heartbeat, tremors, palpitations, etc., which will lead to teratogenic and carcinogenic human body.

【Principles】

Ractopamine ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The ractopamine antigen is precoated on the wells. The precoated antigen compete the ractopamine antibody (Antibody Solution) with ractopamine in the sample, ractopamine antibody binds to the ractopamine Enzyme Conjugate. After the color development of TMB Substrate, the absorbance value of the sample is negatively correlated with the ractopamine contained in it. Compared with the standard curve and multiplied by its corresponding dilution factor, the content of ractopamine in the sample can be calculated.

【Cross Reactivity】

Ractopamine 100%

Dobutamine <1%

Dopamine hydrochloride <1%

Clenbuterol <1%

Salbutamol <1%

【Materials Required But Not Provided】

1. Devices:

—- Microplate Reader (450nm/630nm filter)

—- Constant Temperature Incubator (Optional)

—- Homogenizer

—- Vortex

—- Centrifuge

—- Centrifuge tube: 5mL/7mL, 50mL

—- Graduated Pipette: 10mL

—- Rubber Bulb Syringe

—- Balance (sensibility: 0.01g)

—- Micropipette: Single channel pipette (10-100µL, 100-1000µL)

Multi-channel pipette (30-300µL)

2. Reagent:

—- Deionized Water

—- Methanol (AR)

—- Trichloroacetic Acid (CCl₃COOH) (AR)

—- 18M Concentrated Sulfuric Acid (H₂SO₄)

【Provided Materials and Reagent】

Note: The concentrations of the standards are 0ppb, 0.05ppb, 0.15ppb, 0.45ppb, 1.35ppb, 4.05ppb.