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Animal Tissue Antibiotic Tylosin ELISA Test Kit

Qualitative or quantitative analysis of Tylosin in tissue.

Meizheng Bio-Tech Company Overview. PDF

Background:

Tylosin is a macrolide antibiotic mainly used to prevent livestock and poultry pathogens and mycoplasma infections.

Use Principles: 

Tylosin ELISA Test Kit is an indirect competitive enzyme-labeled immunoassay. The Tylosin antigen is precoated on the wells. The precoated antigen compete the Tylosin antibody (antibody solution) with Tylosin in the sample, anti-Tylosin antibody binds to the Tylosin-HRP enzyme conjugate. Then pipette the substrate solutions to the wells to convert the color.

The color of unknown samples is compared to the color of the standards and the Tylosin concentration of the samples is derived.

ManufacturerMeizheng
Order No.ME60A
AnalyteTylosin
Sample TypeTissue
MethodologyIndirect competitive enzyme-labeled immunoassay.
Packaging96 Wells
StorageThe kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE!
Shelf lifeThe validity for this kit is 12 months
Lead timeShipped within 5-12 working days.
Cross ReactivityTylosin ………………………………………100%
Tilmicosin ……………………………………105%
 Calculate ResultAnalyze the result by using the software
Sensitivity 0.5ppb
Limit of detection20ppb
Percent Recovery, %100±30%
Precision (CV %):Intra-lab assay: CV%≤10%

Provided Materials and Reagent

Component96 wells
Antigen coated plate12 test strips of 8 wells each
6 vials of standard1mL each
Enzyme Conjugate6mL
Antibody Solution6mL
Substrate A6mL
Substrate B6mL
Concentrated Wash Buffer (10×)40mL
Concentrated sample diluting solution40mL
Package Insert1
Testing Report1
Plate Cover Sheet2

Note:The concentrations of the standards are 0ppb, 0.5ppb, 1.5ppb, 4.5ppb, 13.5ppb and 40.5ppb.

Materials Required But Not Provided

  1. Devices:

—- Microwell plate Reader (450nm/630nm)

—- Homogenizer

—- Oscillator

—- Vortex

—- Centrifuge

—- Balance (sensibility: 0.01g)

—- Transfer pipette: 10mL

—- Aurilave

—- Centrifuge tube: 5mL, 7mL, 50mL

—-Micropipette: Single channel pipet (10µL-100µL, 100µL-1000µL)

Multi-channel pipet (30µL -300µL)

  1. Reagent:

—- Deionized water

—- Concentrated sulfuric acid (18 mol/L)

 Note: The pictures shown are sample product pictures.

For the actual product, please check Provided Materials and Reagent or consult the sales manager.

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