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    Animal Tissues, Seafood and Eggs Antibiotic Tetracyclines ELISA Test Kits

    The Tetracyclines ELISA Kit is competitive ELISA for the qualitative or quantitative analysis of tetracycline, oxytetracycline, chlortetracycl- ine, and doxycycline in samples of animal tissues, seafood, and eggs, etc.

    Summary

    Tetracyclines are antibiotics which inhibit the bacterial growth by stopping protein synthesis. They have been widely used for the past forty years as therapeutic agent in human and veterinary medicine but also as growth promoter in animal husbandry. But the abuse of antibiotics will lead to the residues in the livestock and indirectly affect the health of human being.

    This detection method is sensitive, fast, simple, and specific, only need 45mins for operating and a small amount of sampling.

    Order No.: ME09AER

    LOD: 0.2 ppb

    Package: 96T

    Use Principles

    The Tetracyclines ELISA kit is an indirect competitive enzyme-labeled immunoassay. The antigen is precoated on the wells. The precoated antigen compete the antibody (antibody solution) with tetracycline in the sample, anti-tetracycline antibody binds to the HRP enzyme conjugate. Then pipette the substrate solution to the wells to convert the color.

    The color of unknown samples is compared to the color of the standards and the tetracycline concentration of the samples is derived.

    Cross-reactivity

    Tetracycline 100%

    Chlortetracycline 180%

    Oxytetracycline 70%

    Doxycycline 90%

    Materials Required But Not Provided

    Devices:

    ---- Microplate Reader (450nm/630nm filter)

    ---- Homogenizer

    ---- Oscillator

    ---- Vortex

    ---- Centrifuge

    ---- Centrifuge tube: 10mL/7mL, 50mL

    ---- Balance (sensibility: 0.01g)

    ---- Graduated pipette: 10mL

    ---- Micropipette: Single channel pipette (10-100µL, 100-1000µL)

    Multi-channel pipette (30-300µL)

    Reagent:

    ---- Deionized water

    ---- Methanol (AR)

    ---- 18M concentrated sulfuric acid (H2SO4)

    [Provided Materials and Reagent]

    Component96 wells
    Antigen coated plate96T
    6 vials of Standard2mL each
    Enzyme Conjugate6mL
    Antibody Solution6mL
    Substrate A6mL
    Substrate B6mL
    Concentrated Sample Diluting Buffer40mL
    Concentrated Wash Buffer (10×)40mL
    Concentrated sample extracting buffer40mL
    Package Insert1
    Testing Report1
    Plate Cover Sheet2

    Note: The concentrations of the standards are 0ppb, 0.2ppb, 0.6ppb, 1.8ppb, 5.4ppb and 16.2ppb.

    Note: The pictures shown are sample product pictures.

    For the actual product, please check Provided Materials and Reagent or consult the sales manager.

    Quantity

    1-10, 11-100, 101-1000, 1001-10000

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