Background:
Lincomycin is a Lincosamides antibiotic mainly used to restrain synthesis of bacterial protein. Lincomycin residual in livestock and poultry will hurt liver and cause allergic reaction and Cardiovascular reaction.
Use Principles:
Lincomycin ELISA Test Kit is an indirect competitive enzyme-labeled immunoassay. The Lincomycin antigen is precoated on the wells. The precoated antigen compete the Lincomycin antibody (antibody solution) with Lincomycin in the sample, anti- Lincomycin antibody binds to the Lincomycin -HRP enzyme conjugate. Then pipete the substrate solutions to the wells to convert the color.
The color of unknown samples is compared to the color of the standards and the Lincomycin concentration of the samples is derived.
Manufacturer | Meizheng |
Order No. | ME35A |
Analyte | Lincomycin |
Sample Type | Tissue of poultry and seafood. |
Methodology | Indirect competitive enzyme-labeled immunoassay. |
Packaging | 96 Wells |
Storage | The kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE! |
Shelf life | The validity for this kit is 12 months |
Lead time | Shipped within 5-12 working days. |
Cross Reactivity | Clindamycin ………………………………<1% Spiramycin…………………………………<1% Erythromycin………………………………<1% Tylosin………………………………………<1% Streptomycin…………………………………<1% Kanamycin……………………………………<1% Spectinomycin………………………………<1% |
Calculate Result | Analyze the result by using the software |
Sensitivity | 0.2ppb |
Limit of detection | 4ppb |
Percent Recovery, % | 100±20% |
Precision (CV %): | Intra-lab assay: CV%≤10% |
Provided Materials and Reagent
Component | 96 wells |
Antigen coated plate | 12 test strips of 8 wells each |
6 vials of standard | 1mL each |
High concentration standard (2ppm) | 1mL each |
Enzyme Conjugate | 6mL |
Antibody Solution | 6mL |
Substrate A | 6mL |
Substrate B | 6mL |
Concentrated Wash Buffer (10×) | 40mL |
Concentrated sample diluting buffer (2×) | 50mL |
Package Insert | 1 |
Testing Report | 1 |
Plate Cover Sheet | 2 |
6 vials of standard | 1mL each |
Note:The concentrations of the standards are 0ppb, 0.2ppb, 0.6ppb, 1.8ppb, 5.4ppb and 16.2ppb.
Materials Required But Not Provided
- Devices:
—- Microwell plate Reader (450nm/630nm)
—- Homogenizer
—- Oscillator
—- Vortex
—- Centrifuge
—- Balance (sensibility: 0.01g)
—- Transfer pipette: 10mL
—- Aurilave
—- Centrifuge tube: 5mL, 7mL, 50mL
—-Micropipette: Single channel pipet (10µL-100µL, 100µL-1000µL)
Multi-channel pipet (30µL -300µL)
- Reagent:
—- Deionized water
—- Concentrated sulfuric acid (18 mol/L)
Note: The pictures shown are sample product pictures.
For the actual product, please check Provided Materials and Reagent or consult the sales manager.