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    Antibiotic Quinolones ELISA Test Kit for Raw milk, Milk powder and Honey

    The Quinolones ELISA Kit is competitive ELISA for the qualitative or quantitative analysis of quinolones in samples of Raw milk, Milk powder and Honey.

    Summary

    The quinolones are a family of synthetic broad-spectrum antibiotics. Most quinolones belong to the subgroup of fluoroquinolones. Some of these fluoroquinolones are admitted as antibiotics especially for cattle, pigs, chicken, and fish/shrimp farming.

    Order No.: SC0039

    Sensitivity: 0.5 ppb

    Package: 96T

    Samples: Raw milk, Milk powder, Honey

    Use Principles

    The Quinolones ELISA kit is an indirect competitive enzyme-labeled immunoassay. The antigen is precoated on the wells. The precoated antigen compete the antibody (antibody solution) with quinolones in the sample, anti-quinolones antibody binds to the HRP enzyme conjugate. Then pipette the substrate solution to the wells to convert the color.

    The color of unknown samples is compared to the color of the standards and the quinolones concentration of the samples is derived.

    Cross-reactivity

    Enrofloxacin (ENR) 100%

    Ciprofloxacin (CIP) 25%

    Nadifloxacin (NAD) 323%

    Enoxacin (ENO)  37%

    Lomefloxacin (LOM) 21%

    Norfloxacin (NOR) 258%

    Marbolxacin (MAR) 19%

    Pefloxacin (PEF) 135%

    Ofloxacin (OFL) 55%

    Danofloxain (DAN) 69%

    Orbifloxacin(ORB) 17%

    Sarafloxacin (SAR) 33%

    Oxolinic acid (OXO) 51%

    Flumequine (FLU) 143%

    Materials Required But Not Provided

    1. Devices:

    ---- Microplate Reader (450nm/630nm filter)

    ---- Homogenizer

    ---- Vortex

    ---- Centrifuge

    ---- Centrifuge tube: 10mL/7mL, 50mL

    ---- Constant temperature incubator

    ---- Balance (sensibility: 0.01g)

    ---- Termovap Sample Concentrator

    ---- Water bath

    ---- Graduated pipette: 10mL

    ---- Micropipette: Single channel pipette (10-100µL, 100-1000µL)

    Multi-channel pipette (30-300µL)

    1. Reagent:

    ---- Acetonitrile (AR)

    ---- Dichloromethane (AR)

    ---- N-hexane (AR)

    ---- Deionized water

    ---- Trichloroacetic acid (CCl3COOH) (AR)

    ---- 18M concentrated sulfuric acid (H2SO4)

    [Provided Materials and Reagent]

    Component96 wells
    Antigen coated plate96T
    6 vials of Standard1mL each
    High concentration standard (1ppm)1mL
    Enzyme Conjugate6mL
    Antibody Solution6mL
    Substrate A6mL
    Substrate B6mL
    Concentrated Wash Buffer (10×)40mL
    Concentrated Sample Diluting Buffer I (8×)15mL
    Concentrated Sample Diluting Buffer II (4×)40mL
    Package Insert1
    Testing Report1
    Plate Cover Sheet2

    Note: The concentrations of the standards are 0ppb, 0.2ppb, 0.6ppb, 1.8ppb, 5.4ppb, and 16.2ppb.

    Note: The pictures shown are sample product pictures.

    For the actual product, please check Provided Materials and Reagent or consult the sales manager.

    Quantity

    1-10, 11-100, 101-1000, 1001-10000

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