The quinolones are a family of synthetic broad-spectrum antibiotics. Most quinolones belong to the subgroup of fluoroquinolones. Some of these fluoroquinolones are admitted as antibiotics especially for cattle, pigs, chicken, and fish/shrimp farming.
Order No.: SC0039
Sensitivity: 0.5 ppb
Samples: Raw milk, Milk powder, Honey
The Quinolones ELISA kit is an indirect competitive enzyme-labeled immunoassay. The antigen is precoated on the wells. The precoated antigen compete the antibody (antibody solution) with quinolones in the sample, anti-quinolones antibody binds to the HRP enzyme conjugate. Then pipette the substrate solution to the wells to convert the color.
The color of unknown samples is compared to the color of the standards and the quinolones concentration of the samples is derived.
Enrofloxacin (ENR) 100%
Ciprofloxacin (CIP) 25%
Nadifloxacin (NAD) 323%
Enoxacin (ENO) 37%
Lomefloxacin (LOM) 21%
Norfloxacin (NOR) 258%
Marbolxacin (MAR) 19%
Pefloxacin (PEF) 135%
Ofloxacin (OFL) 55%
Danofloxain (DAN) 69%
Sarafloxacin (SAR) 33%
Oxolinic acid (OXO) 51%
Flumequine (FLU) 143%
Materials Required But Not Provided
—- Microplate Reader (450nm/630nm filter)
—- Centrifuge tube: 10mL/7mL, 50mL
—- Constant temperature incubator
—- Balance (sensibility: 0.01g)
—- Termovap Sample Concentrator
—- Water bath
—- Graduated pipette: 10mL
—- Micropipette: Single channel pipette (10-100µL, 100-1000µL)
Multi-channel pipette (30-300µL)
—- Acetonitrile (AR)
—- Dichloromethane (AR)
—- N-hexane (AR)
—- Deionized water
—- Trichloroacetic acid (CCl3COOH) (AR)
—- 18M concentrated sulfuric acid (H2SO4)
[Provided Materials and Reagent]
|Antigen coated plate||96T|
|6 vials of Standard||1mL each|
|High concentration standard (1ppm)||1mL|
|Concentrated Wash Buffer (10×)||40mL|
|Concentrated Sample Diluting Buffer I (8×)||15mL|
|Concentrated Sample Diluting Buffer II (4×)||40mL|
|Plate Cover Sheet||2|
Note: The concentrations of the standards are 0ppb, 0.2ppb, 0.6ppb, 1.8ppb, 5.4ppb, and 16.2ppb.
Note: The pictures shown are sample product pictures.
For the actual product, please check Provided Materials and Reagent or consult the sales manager.