The Quinolones ELISA kit is an indirect competitive enzyme-labeled immunoassay. The antigen is precoated on the wells. The precoated antigen compete the antibody (antibody solution) with quinolones in the sample, anti-quinolones antibody binds to the HRP enzyme conjugate. Then pipette the substrate solution to the wells to convert the color.
The color of unknown samples is compared to the color of the standards and the quinolones concentration of the samples is derived.
【Scope of Application】
The Quinolones ELISA Kit is competitive ELISA for the qualitative or quantitative analysis of quinolones in shrimp.
Enrofloxacin (ENR) 100%
Ciprofloxacin (CIP) ＞100%
Norfloxacin (NOR) ＞100%
Ofloxacin (OFL) 43%
Sarafloxacin (SAR) 36%
Pefloxacin (PEF) ＞100%
Lomefloxacin (LOM) 46%
Danofloxain (DAN) ＞100%
Enoxacin (ENO) ＞100%
Flumequine (FLU) 57%
Nadifloxacin (NAD) 37%
Oxolinic acid (OXO) ＞100%
Marbolxacin (MAR) 23%
Difloxacin (DIF) 54%
【Materials Required But Not Provided】
—- Microplate Reader (450nm/630nm filter)
—- Constant Temperature Incubator
—- Balance (sensibility: 0.01g)
—- Rubber Bulb Syringe
—- Graduated pipette: 10mL
—- Micropipette: Single channel pipette (10-100µL, 100-1000µL)
Multi-channel pipette (30-300µL)
—- Deionized water
【Provided Materials and Reagent】
|Antigen coated plate||96T|
|6 vials of Standard||1mL each|
|High concentration standard (1ppm)||1mL|
|Concentrated Wash Buffer (10×)||40mL|
|Concentrated Sample Diluting Buffer||40mL|
|Plate Cover Sheet||2|
Note: The concentrations of the standards are 0ppb, 0.05ppb, 0.15ppb, 0.45ppb, 1.35ppb, and 4.05ppb.
Note: The pictures shown are sample product pictures.
For the actual product, please check Provided Materials and Reagent or consult the sales manager.