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Antibiotic Tilmicosin ELISA Test Kit for Tissue

Qualitative or quantitative analysis of Tilmicosin in tissue.

Meizheng Bio-Tech Company Overview. PDF

Background:

Tilmicosin is a macrolide antibiotic mainly used to prevent livestock and poultry pathogens and mycoplasma infections.

Use Principles: 

Tilmicosin ELISA Test Kit is an indirect competitive enzyme-labeled immunoassay. The Tilmicosin antigen is precoated on the wells. The precoated antigen compete the Tilmicosin antibody (antibody solution) with Tilmicosin in the sample, anti-Tilmicosin antibody binds to the Tilmicosin-HRP enzyme conjugate. Then pipette the substrate solutions to the wells to convert the color.

The color of unknown samples is compared to the color of the standards and the Tilmicosin concentration of the samples is derived.

ManufacturerMeizheng
Order No.ME41A
AnalyteTilmicosin
Sample TypeTissue
MethodologyIndirect competitive enzyme-labeled immunoassay.
Packaging96 Wells
StorageThe kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE!
Shelf lifeThe validity for this kit is 12 months
Lead timeShipped within 5-12 working days.
Cross ReactivityTilmicosin ……………………………………100%
Tylosin ………………………………………<1%
Spectinomycin, lincomycin………………<0.1%
Calculate ResultAnalyze the result by using the software
Sensitivity 0.5ppb
Limit of detection10ppb
Percent Recovery, %100±30%
Precision (CV %):Intra-lab assay: CV%≤10%

Provided Materials and Reagent

Component96 wells
Antigen coated plate12 test strips of 8 wells each
6 vials of standard1mL each
Enzyme Conjugate6mL
Antibody Solution6mL
Substrate A6mL
Substrate B6mL
Concentrated Wash Buffer (10×)40mL
Concentrated sample diluting buffer40mL
Package Insert1
Testing Report1
Plate Cover Sheet2

Note:The concentrations of the standards are 0ppb, 0.5ppb, 1.5ppb, 4.5ppb, 13.5ppb and 40.5ppb.

Materials Required But Not Provided

  1. Devices:

—- Microwell plate Reader (450nm/630nm)

—- Homogenizer

—- Oscillator

—- Vortex

—- Centrifuge

—- Balance (sensibility: 0.01g)

—- Transfer pipette: 10mL

—- Aurilave

—- Centrifuge tube: 5mL, 7mL, 50mL

—-Micropipette: Single channel pipet (10µL-100µL, 100µL-1000µL)

Multi-channel pipet (30µL -300µL)

  1. Reagent:

—- Deionized water

—- Concentrated sulfuric acid (18 mol/L)

 Note: The pictures shown are sample product pictures.

For the actual product, please check Provided Materials and Reagent or consult the sales manager.

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