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    Antibiotic Tilmicosin ELISA Test Kit for Tissue

    Qualitative or quantitative analysis of Tilmicosin in tissue.

    Meizheng Bio-Tech Company Overview. PDF

    Background:

    Tilmicosin is a macrolide antibiotic mainly used to prevent livestock and poultry pathogens and mycoplasma infections.

    Use Principles: 

    Tilmicosin ELISA Test Kit is an indirect competitive enzyme-labeled immunoassay. The Tilmicosin antigen is precoated on the wells. The precoated antigen compete the Tilmicosin antibody (antibody solution) with Tilmicosin in the sample, anti-Tilmicosin antibody binds to the Tilmicosin-HRP enzyme conjugate. Then pipette the substrate solutions to the wells to convert the color.

    The color of unknown samples is compared to the color of the standards and the Tilmicosin concentration of the samples is derived.

    ManufacturerMeizheng
    Order No.ME41A
    AnalyteTilmicosin
    Sample TypeTissue
    MethodologyIndirect competitive enzyme-labeled immunoassay.
    Packaging96 Wells
    StorageThe kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE!
    Shelf lifeThe validity for this kit is 12 months
    Lead timeShipped within 5-12 working days.
    Cross ReactivityTilmicosin ……………………………………100%
    Tylosin ………………………………………<1%
    Spectinomycin, lincomycin……………...<0.1%
    Calculate ResultAnalyze the result by using the software
    Sensitivity 0.5ppb
    Limit of detection10ppb
    Percent Recovery, %100±30%
    Precision (CV %):Intra-lab assay: CV%≤10%

    Provided Materials and Reagent

    Component96 wells
    Antigen coated plate12 test strips of 8 wells each
    6 vials of standard1mL each
    Enzyme Conjugate6mL
    Antibody Solution6mL
    Substrate A6mL
    Substrate B6mL
    Concentrated Wash Buffer (10×)40mL
    Concentrated sample diluting buffer40mL
    Package Insert1
    Testing Report1
    Plate Cover Sheet2

    Note:The concentrations of the standards are 0ppb, 0.5ppb, 1.5ppb, 4.5ppb, 13.5ppb and 40.5ppb.

    Materials Required But Not Provided

    1. Devices:

    ---- Microwell plate Reader (450nm/630nm)

    ---- Homogenizer

    ---- Oscillator

    ---- Vortex

    ---- Centrifuge

    ---- Balance (sensibility: 0.01g)

    ---- Transfer pipette: 10mL

    ---- Aurilave

    ---- Centrifuge tube: 5mL, 7mL, 50mL

    ----Micropipette: Single channel pipet (10µL-100µL, 100µL-1000µL)

    Multi-channel pipet (30µL -300µL)

    1. Reagent:

    ---- Deionized water

    ---- Concentrated sulfuric acid (18 mol/L)

     Note: The pictures shown are sample product pictures.

    For the actual product, please check Provided Materials and Reagent or consult the sales manager.

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