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    Antibiotic Tylosin ELISA Test Kit for Animal Tissue

    Qualitative or quantitative analysis of Tylosin in tissue.

    Summary

    Tylosin is a macrolide antibiotic mainly used to prevent livestock and poultry pathogens and mycoplasma infections.

    Order No.: ME60AER

    LOD: 0.5 ppb

    Package: 96T

    Use Principles

    Tylosin ELISA Test Kit is an indirect competitive enzyme-labeled immunoassay. The Tylosin antigen is precoated on the wells. The precoated antigen compete the Tylosin antibody (antibody solution) with Tylosin in the sample, anti-Tylosin antibody binds to the Tylosin-HRP enzyme conjugate. Then pipette the substrate solutions to the wells to convert the color.

    The color of unknown samples is compared to the color of the standards and the Tylosin concentration of the samples is derived.

    Cross-reactivity

    Tylosin …………………………………………………100%

    Tilmicosin ……………………………………………105%

    Materials Required But Not Provided

    1. Devices:

    ---- Microwell plate Reader (450nm/630nm)

    ---- Homogenizer

    ---- Oscillator

    ---- Vortex

    ---- Centrifuge

    ---- Balance (sensibility: 0.01g)

    ---- Transfer pipette: 10mL

    ---- Aurilave

    ---- Centrifuge tube: 5mL, 7mL, 50mL

    ----Micropipette: Single channel pipet (10µL-100µL, 100µL-1000µL)

    Multi-channel pipet (30µL -300µL)

    1. Reagent:

    ---- Deionized water

    ---- Concentrated sulfuric acid (18 mol/L)

    Provided Materials and Reagent

    Component96 wells
    Antigen coated plate12 test strips of 8 wells each
    6 vials of standard1mL each
    Enzyme Conjugate6mL
    Antibody Solution6mL
    Substrate A6mL
    Substrate B6mL
    Concentrated Wash Buffer (10×)40mL
    Concentrated sample diluting solution40mL
    Package Insert1
    Testing Report1
    Plate Cover Sheet2

    Note: The concentrations of the standards are 0ppb, 0.5ppb, 1.5ppb, 4.5ppb, 13.5ppb and 40.5ppb.

    Note: The pictures shown are sample product pictures.

    For the actual product, please check Provided Materials and Reagent or consult the sales manager.

    Quantity

    1-10, 11-100, 101-1000, 1001-10000

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