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Ciprofloxacin ELISA Test Kit for Animal Tissue and Egg

【Summary】

Ciprofloxacin is a fluoroquinolone antibiotic used to treat a number of bacterial infections. It is a bactericidal agent. Ciprofloxacin is active against some Gram-positive and many Gram-negative bacteria.

【Principles】

The Ciprofloxacin ELISA kit is an indirect competitive enzyme-labeled immunoassay. The antigen is precoated on the wells. The precoated antigen compete the antibody (antibody solution) with Ciprofloxacin in the sample, anti-ciprofloxacin antibody binds to the HRP enzyme conjugate. Then pipette the substrate solution to the wells to convert the color.

The color of unknown samples is compared to the color of the standards and the ciprofloxacin concentration of the samples is derived.

【Scope of Application】

The Ciprofloxacin ELISA Kit is competitive ELISA for the qualitative or quantitative analysis of ciprofloxacin in samples of animal tissues, eggs, etc.

【Cross Reactivity】

Ciprofloxacin (CIP) 100%

【Materials Required But Not Provided】

  1. Devices:

—- Microplate Reader (450nm/630nm filter)

—- Vortex

—- Constant Temperature Incubator

—- Centrifuge

—- Homogenizer

—- Balance (sensibility: 0.01g)

—- Rubber Bulb Syringe

—- Graduated pipette: 10mL

—- Micropipette: Single channel pipette (10-100µL, 100-1000µL)

Multi-channel pipette (30-300µL)

  1. Reagent:

—- Deionized water

—- 18M concentrated sulfuric acid (H2SO4)

【Provided Materials and Reagent】

Component 96 wells
Antigen coated plate96T
5 vials of Standard1mL each
High concentration standard (1ppm)1mL
Enzyme Conjugate6mL
Antibody Solution6mL
Substrate A6mL
Substrate B6mL
Concentrated Wash Buffer (10×)40mL
Concentrated Sample Diluting Buffer40mL
Concentrated Sample extracting Buffer (15×)60mL
Package Insert1
Testing Report1
Plate Cover Sheet2

Note: The concentrations of the standards are 0ppb, 0.05ppb, 0.15ppb, 0.45ppb, and 1.35ppb.

 Note: The pictures shown are sample product pictures.

For the actual product, please check Provided Materials and Reagent or consult the sales manager.

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