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    Ciprofloxacin ELISA Test Kit for Animal Tissue and Egg

    Order No.: ME11BER

    Scope of Application

    The Ciprofloxacin ELISA Kit is competitive ELISA for the qualitative or quantitative analysis of ciprofloxacin in samples of animal tissues, eggs, etc.

     

    Summary

    Ciprofloxacin is a fluoroquinolone antibiotic used to treat a number of bacterial infections. It is a bactericidal agent. Ciprofloxacin is active against some Gram-positive and many Gram-negative bacteria.

    Principles

    The Ciprofloxacin ELISA kit is an indirect competitive enzyme-labeled immunoassay. The antigen is precoated on the wells. The precoated antigen compete the antibody (antibody solution) with Ciprofloxacin in the sample, anti-ciprofloxacin antibody binds to the HRP enzyme conjugate. Then pipette the substrate solution to the wells to convert the color.

    The color of unknown samples is compared to the color of the standards and the ciprofloxacin concentration of the samples is derived.

    Cross Reactivity

    Ciprofloxacin (CIP) 100%

    Materials Required But Not Provided

    1. Devices:

    ---- Microplate Reader (450nm/630nm filter)

    ---- Vortex

    ---- Constant Temperature Incubator

    ---- Centrifuge

    ---- Homogenizer

    ---- Balance (sensibility: 0.01g)

    ---- Rubber Bulb Syringe

    ---- Graduated pipette: 10mL

    ---- Micropipette: Single channel pipette (10-100µL, 100-1000µL) Multi-channel pipette (30-300µL)

    2. Reagent:

    ---- Deionized water

    ---- 18M concentrated sulfuric acid (H2SO4)

    【Provided Materials and Reagent】

    Component96 wells
    Antigen coated plate96T
    5 vials of Standard1mL each
    High concentration standard (1ppm)1mL
    Enzyme Conjugate6mL
    Antibody Solution6mL
    Substrate A6mL
    Substrate B6mL
    Concentrated Wash Buffer (10×)40mL
    Concentrated Sample Diluting Buffer40mL
    Concentrated Sample extracting Buffer (15×)60mL
    Package Insert1
    Testing Report1
    Plate Cover Sheet2

    Note: The concentrations of the standards are 0ppb, 0.05ppb, 0.15ppb, 0.45ppb, and 1.35ppb.

    Quantity

    1-10, 11-100, 101-1000, 1001-10000

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