Ciprofloxacin is a fluoroquinolone antibiotic used to treat a number of bacterial infections. It is a bactericidal agent. Ciprofloxacin is active against some Gram-positive and many Gram-negative bacteria.
The Ciprofloxacin ELISA kit is an indirect competitive enzyme-labeled immunoassay. The antigen is precoated on the wells. The precoated antigen compete the antibody (antibody solution) with Ciprofloxacin in the sample, anti-ciprofloxacin antibody binds to the HRP enzyme conjugate. Then pipette the substrate solution to the wells to convert the color.
The color of unknown samples is compared to the color of the standards and the ciprofloxacin concentration of the samples is derived.
Ciprofloxacin (CIP) 100%
【Materials Required But Not Provided】
---- Microplate Reader (450nm/630nm filter)
---- Constant Temperature Incubator
---- Balance (sensibility: 0.01g)
---- Rubber Bulb Syringe
---- Graduated pipette: 10mL
---- Micropipette: Single channel pipette (10-100µL, 100-1000µL) Multi-channel pipette (30-300µL)
---- Deionized water
---- 18M concentrated sulfuric acid (H2SO4)
【Provided Materials and Reagent】
|Antigen coated plate||96T|
|5 vials of Standard||1mL each|
|High concentration standard (1ppm)||1mL|
|Concentrated Wash Buffer (10×)||40mL|
|Concentrated Sample Diluting Buffer||40mL|
|Concentrated Sample extracting Buffer (15×)||60mL|
|Plate Cover Sheet||2|
Note: The concentrations of the standards are 0ppb, 0.05ppb, 0.15ppb, 0.45ppb, and 1.35ppb.