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    Summary

    Colistin sulfate is an antibacterial spectrum drug. It has antibacterial effect on Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, Haemophilus, enterobacter, Salmonella, Shigella, pertussis, pasteurella and Vibrio.

    The kit is a detection product developed by using ELISA technology. It has the advantages of fast, simple, accurate and high sensitivity. The operation time is only 45 minutes, which can minimize the operation error and work intensity.

    Use Principles

    Colistin Sulfate ELISA Kit is an indirect competitive enzyme-labeled immunoassay. The colistin sulfate antigen is precoated on the wells. The precoated antigen compete the colistin sulfate antibody (antibody solution) with colistin sulfate in the sample, anti-colistin sulfate antibody binds to the colistin sulfate-HRP enzyme conjugate. Then pipette the substrate solutions to the wells to develop the color.

    Compare the color of tested samples to the color of the standards to get the colistin sulfate concentration of the samples.

    Scope of Application

    Qualitative or quantitative analysis of colistin sulfate in animal tissues.

    Cross-reactivity

    Colistin Sulfate 100%

    Materials Required But Not Provided

    1. Devices:

    ---- Microwell Plate Reader (450nm/630nm)

    ---- Homogenizer

    ---- Vortex

    ---- Centrifuge

    ---- Balance (sensibility: 0.01g)

    ---- Graduated pipette: 10mL

    ---- Rubber bulb

    ---- Centrifuge tube: 5mL or 7mL, 50mL

    ---- Micropipette: Single channel (10-100µL, 100-1000µL)

    Multichannel (30-300µL)

    1. Reagent:

    ---- Deionized water

    ---- Concentrated sulfuric acid (18mol/L)

    Provided Materials and Reagent

    Component96 wells
    Antigen coated plate12 test strips of 8 wells each
    6 vials of standard1mL each
    Enzyme Conjugate6mL
    Antibody Solution6mL
    Substrate A6mL
    Substrate B6mL
    Concentrated Wash Buffer (10×)40mL
    Concentrated Sample Diluting Buffer40mL
    Instruction1
    Test report1
    Cover film2

    Note: The concentrations of the standards are 0ppb, 1ppb, 3ppb, 9ppb, 27ppb and 81ppb.

     Note: The pictures shown are sample product pictures.

    For the actual product, please check Provided Materials and Reagent or consult the sales manager.

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